PMID- 22401906
OWN - NLM
STAT- MEDLINE
DCOM- 20120507
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 891-892
DP  - 2012 Apr 1
TI  - Rapid determination of endogenous cytokinins in plant samples by combination of 
      magnetic solid phase extraction with hydrophilic interaction 
      chromatography-tandem mass spectrometry.
PG  - 27-35
LID - 10.1016/j.jchromb.2012.02.015 [doi]
AB  - A 2-acrylamido-2-methyl-1-propanesulfonic acid-co-ethylene glycol dimethacrylate 
      (Fe(3)O(4)/SiO(2)/P(AMPS-co-EGDMA)) copolymer was prepared and used as a magnetic solid 
      phase extraction (MSPE) medium for recovery of endogenous cytokinins (CKs) from 
      plant extracts. This magnetic porous polymer was characterized by electron 
      microscopy, nitrogen sorption experiments, elemental analysis and 
      Fourier-transformed infrared spectroscopy. It was demonstrated to have high 
      extraction capacity toward CKs in plants due to its specificity, surface area and 
      porous structure. Coupled with hydrophilic interaction chromatography-tandem mass 
      spectrometry (HILIC-MS/MS), a rapid, simple, and effective MSPE-HILIC-MS/MS 
      analytical method for the quantitative analysis of endogenous CKs in Oryza sativa 
      (O. sativa) roots was successfully established. Good linearities were obtained 
      for all CKs investigated with correlation coefficients (R(2)>0.9975. The results 
      showed that LODs (S/N=3) were ranged from 0.18 to 3.65 pg mL(-)(1). Reproducibility 
      of the method was obtained with intra-day and inter-day relative standard 
      deviations (RSDs) less than 16.1% and the recoveries in plant samples ranged from 
      72.8% to 115.5%. Finally, the MSPE-HILIC-MS/MS method was applied to several 
      plant samples, and the amounts of endogenous CKs in O. sativa roots, leaves and 
      Arabidopsis thaliana (A. thaliana) were successfully determined.
CI  - Copyright A(c) 2012 Elsevier B.V. All rights reserved.
FAU - Liu, Zhao
AU  - Liu Z
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Cai, Bao-Dong
AU  - Cai BD
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20120218
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Cytokinins)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Cytokinins/*analysis
MH  - Microscopy, Electron, Scanning
MH  - Plants/*chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/*methods
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/03/10 06:00
MHDA- 2012/05/09 06:00
CRDT- 2012/03/10 06:00
PHST- 2011/11/29 00:00 [received]
PHST- 2012/02/08 00:00 [revised]
PHST- 2012/02/12 00:00 [accepted]
PHST- 2012/03/10 06:00 [entrez]
PHST- 2012/03/10 06:00 [pubmed]
PHST- 2012/05/09 06:00 [medline]
AID - S1570-0232(12)00103-1 [pii]
AID - 10.1016/j.jchromb.2012.02.015 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 1;891-892:27-35. doi: 
      10.1016/j.jchromb.2012.02.015. Epub 2012 Feb 18.