PMID- 2240357
OWN - NLM
STAT- MEDLINE
DCOM- 19901204
LR  - 20190820
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 14
IP  - 11
DP  - 1990 Nov
TI  - Identification of a mislabeled fixed specimen by DNA analysis.
PG  - 1076-8
AB  - Formalin-fixed, paraffin embedded surgical specimens can be identified by 
      genotypic analysis. DNA was extracted from single sections of fixed tissue and 
      amplified by the polymerase chain reaction for the human leukocyte antigen (HLA) 
      DQ alpha and low density lipoprotein receptor genes. These two loci are 
      polymorphic and fixed specimens can be distinguished based on differences between 
      these alleles. The examination can be performed in 2 days and the HLA DQ alpha 
      test utilized a nonisotopic color detection method. In this case report, a 
      mislabeled specimen was identified as a section from a mastectomy because they 
      had identical genotypes. Molecular genetic analysis of the DNA present in fixed 
      specimens can yield information not otherwise evident from the histology.
FAU - Shibata, D
AU  - Shibata D
AD  - Department of Pathology, Los Angeles County-University of Southern California 
      Medical Center 90033.
FAU - Namiki, T
AU  - Namiki T
FAU - Higuchi, R
AU  - Higuchi R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (HLA-DQ Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Breast/*chemistry
MH  - DNA/*analysis
MH  - Female
MH  - Genes/genetics
MH  - HLA-DQ Antigens/analysis
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - *Specimen Handling
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
AID - 10.1097/00000478-199011000-00009 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 1990 Nov;14(11):1076-8. doi: 10.1097/00000478-199011000-00009.