PMID- 22403876
OWN - NLM
STAT- MEDLINE
DCOM- 20130815
LR  - 20181201
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 26
IP  - 2
DP  - 2012 Feb
TI  - [Bio-characteristics of c-kit+ human amniotic fluid-derived mesenchymal stem 
      cells and their differentiation into cardiomyocytes in vitro].
PG  - 152-7
AB  - OBJECTIVE: To investigate the bio-characteristics of c-kit+ human amniotic 
      fluid-derived mesenchymal stem cells (HAFMSCs) and its capacity to differentiate 
      into cardiomyocytes in vitro. METHODS: Fifty samples of human amniotic fluid were 
      obtained from amniocenteses or voluntary termination of pregnancy and were 
      expanded in vitro. c-kit+ HAFMSCs were sorted by flow cytometry and were 
      recultured in the same media. c-kit+ HAFMSCs were amplified and identified, then 
      exposed to osteogenic, adipogenic, and myogenic media. The flow cytometry, and 
      immunocytochemistry were used for identifying the cell phenotype, von Kossa 
      staining for osteogenic differentiation, oil red O staining for adipogenic 
      differentiation, and real-time fluorescent quantitative PCR for the expressions 
      of NKx2.5, Tbx5, GATA-4, and alpha-MHC genes. RESULTS: After the selection 
      procedure, the percentage of c-kit+ HAFMSCs was 3.07% +/- 1.03% of the total 
      adherent cells. The cells expressed MSCs markers (CD29, CD44, CD73, CD90, CD105), 
      and did not express hematopoietic stem cells markers (CD34, CD45). The cells were 
      positive for human leukocyte antigen (HLA)-ABC, and negative for HLA-DR. They 
      also expressed Oct-4, which characterized the undifferentiated stem cell state. 
      The growth curves of c-kit+ HAMFSCs at passages 5, 10, and 15 were similar. Lipid 
      droplet was observed by oil red O staining and calcium deposition by von Kossa 
      staining in the cells at 21 days after adipogenic and osteogenic induction. The 
      myocardium special gene expressions of Tbx5, Nkx2.5, GATA-4, and alpha-MHC were 
      significantly stronger after myogenic induction than those before myogenic 
      induction (P < 0.05). CONCLUSION: Selected c-kit+ HAFMSCs by flow cytometry is a 
      group of pure MSCs, which has potential to differentiate into cardiomyocytes and 
      can be used as seeding cells for myocardium regeneration treatment.
FAU - Bai, Jing
AU  - Bai J
AD  - Department of Cardiology, the First Affiliated Hospital of General Hospital of 
      Chinese PLA, Beijing, 100048, PR China.
FAU - Wang, Yiru
AU  - Wang Y
FAU - Liu, Lifeng
AU  - Liu L
FAU - Chen, Jie
AU  - Chen J
FAU - Wang, Yu
AU  - Wang Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Myocytes, Cardiac/*cytology
MH  - Pregnancy
EDAT- 2012/03/13 06:00
MHDA- 2013/08/16 06:00
CRDT- 2012/03/13 06:00
PHST- 2012/03/13 06:00 [entrez]
PHST- 2012/03/13 06:00 [pubmed]
PHST- 2013/08/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 Feb;26(2):152-7.