PMID- 22405721
OWN - NLM
STAT- MEDLINE
DCOM- 20130208
LR  - 20120910
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 193
IP  - 2
DP  - 2012 Aug
TI  - Prevalence of latent alpha-herpesviruses in Thoroughbred racing horses.
PG  - 579-82
LID - 10.1016/j.tvjl.2012.01.030 [doi]
AB  - The objective of this study was to detect and characterize latent equine herpes 
      virus (EHV)-1 and -4 from the submandibular (SMLN) and bronchial lymph (BLN) 
      nodes, as well as from the trigeminal ganglia (TG) of 70 racing Thoroughbred 
      horses submitted for necropsy following sustaining serious musculoskeletal 
      injuries while racing. A combination of nucleic acid precipitation and 
      pre-amplification steps was used to increase analytical sensitivity. Tissues were 
      deemed positive for latent EHV-1 and/or -4 infection when found PCR positive for 
      the corresponding glycoprotein B (gB) gene in the absence of detectable late 
      structural protein gene (gB gene) mRNA. The EHV-1 genotype was also determined 
      using a discriminatory real-time PCR assay targeting the DNA polymerase gene (ORF 
      30). Eighteen (25.7%) and 58 (82.8%) horses were PCR positive for the gB gene of 
      EHV-1 and -4, respectively, in at least one of the three tissues sampled. Twelve 
      horses were dually infected with EHV-1 and -4, two carried a latent neurotropic 
      strain of EHV-1, six carried a non-neurotropic genotype of EHV-1 and 10 were 
      dually infected with neurotropic and non-neurotropic EHV-1. The distribution of 
      latent EHV-1 and -4 infection varied in the samples, with the TG found to be most 
      commonly infected. Overall, non-neurotropic strains were more frequently detected 
      than neurotropic strains, supporting the general consensus that non-neurotropic 
      strains are more prevalent in horse populations, and hence the uncommon 
      occurrence of equine herpes myeloencephalopathy.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Pusterla, Nicola
AU  - Pusterla N
AD  - Department of Veterinary Medicine and Epidemiology, School of Veterinary 
      Medicine, University of California, Davis, CA 95616, USA. npusterla@ucdavis.edu
FAU - Mapes, Samantha
AU  - Mapes S
FAU - David Wilson, W
AU  - David Wilson W
LA  - eng
PT  - Journal Article
DEP - 20120310
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (DNA, Viral)
SB  - IM
CIN - Vet J. 2012 Aug;193(2):309-10. PMID: 22738739
MH  - Animals
MH  - Bronchi/virology
MH  - California/epidemiology
MH  - DNA, Viral/genetics
MH  - Female
MH  - Herpesviridae Infections/epidemiology/*veterinary/virology
MH  - Herpesvirus 1, Equid/isolation & purification/*physiology
MH  - Herpesvirus 4, Equid/isolation & purification/*physiology
MH  - Horse Diseases/*epidemiology/virology
MH  - Horses
MH  - Lymph Nodes/*virology
MH  - Male
MH  - Mandible/virology
MH  - Pedigree
MH  - Prevalence
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Trigeminal Ganglion/virology
MH  - *Virus Latency
EDAT- 2012/03/13 06:00
MHDA- 2013/02/09 06:00
CRDT- 2012/03/13 06:00
PHST- 2011/06/26 00:00 [received]
PHST- 2012/01/26 00:00 [revised]
PHST- 2012/01/29 00:00 [accepted]
PHST- 2012/03/13 06:00 [entrez]
PHST- 2012/03/13 06:00 [pubmed]
PHST- 2013/02/09 06:00 [medline]
AID - S1090-0233(12)00066-4 [pii]
AID - 10.1016/j.tvjl.2012.01.030 [doi]
PST - ppublish
SO  - Vet J. 2012 Aug;193(2):579-82. doi: 10.1016/j.tvjl.2012.01.030. Epub 2012 Mar 10.