PMID- 22405891
OWN - NLM
STAT- MEDLINE
DCOM- 20120820
LR  - 20120402
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 514
IP  - 1
DP  - 2012 Apr 11
TI  - Recellularized nerve allografts with differentiated mesenchymal stem cells 
      promote peripheral nerve regeneration.
PG  - 96-101
LID - 10.1016/j.neulet.2012.02.066 [doi]
AB  - Chemical-extracted acellular nerve allografting, containing the natural nerve 
      structure and elementary nerve extracellular matrix (ECM), has been used for 
      peripheral nerve-defect treatment experimentally and clinically. However, 
      functional outcome with acellular nerve allografting decreases with increased 
      size of gap in nerve defects. Cell-based therapy is a good strategy for repairing 
      long nerve defects. Bone-marrow-derived mesenchymal stem cells (BMSCs) and 
      adipose-derived mesenchymal stem cells (ADSCs) can be induced to differentiate 
      into cells with Schwann cell-like properties (BMSC-SCs or ADSC-SCs), which have 
      myelin-forming ability in vitro and secrete trophic nerve growth factors. Here, 
      we aimed to determine whether BMSC-SCs or ADSC-SCs are a promising cell type for 
      enriching acellular grafts in nerve repair. We evaluated axonal regeneration 
      distance by immunofluorescence staining after 2-week implantation. We used 
      functional and histomorphometric analysis to evaluate 3-month regeneration of the 
      novel cell-supplemented tissue-engineered nerve graft used to bridge a 15-mm-long 
      sciatic nerve gap in rats. Introducing BMSC-SCs or ADSC-SCs to the acellular 
      nerve graft promoted sciatic nerve regeneration and functional recovery. Nerve 
      regeneration with BMSC-SCs or ADSC-SCs was comparable to that with autografting 
      and Schwann cells alone and better than that with acellular nerve allografting 
      alone. Differentiated bone-marrow-or adipose-derived MSCs may be a promising cell 
      source for tissue-engineered nerve grafts and promote functional recovery after 
      peripheral nerve injury.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd. All rights reserved.
FAU - Wang, Yu
AU  - Wang Y
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Fuxing Road 28, Haidian 
      District, Beijing 100853, China.
FAU - Zhao, Zhe
AU  - Zhao Z
FAU - Ren, Zhiwu
AU  - Ren Z
FAU - Zhao, Bin
AU  - Zhao B
FAU - Zhang, Li
AU  - Zhang L
FAU - Chen, Jifeng
AU  - Chen J
FAU - Xu, Wenjing
AU  - Xu W
FAU - Lu, Shibi
AU  - Lu S
FAU - Zhao, Qing
AU  - Zhao Q
FAU - Peng, Jiang
AU  - Peng J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120303
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
SB  - IM
MH  - Animals
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Nerve Regeneration/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Recovery of Function/physiology
MH  - Sciatic Nerve/*injuries/transplantation
MH  - Transplantation, Autologous
EDAT- 2012/03/13 06:00
MHDA- 2012/08/21 06:00
CRDT- 2012/03/13 06:00
PHST- 2011/11/23 00:00 [received]
PHST- 2012/01/30 00:00 [revised]
PHST- 2012/02/18 00:00 [accepted]
PHST- 2012/03/13 06:00 [entrez]
PHST- 2012/03/13 06:00 [pubmed]
PHST- 2012/08/21 06:00 [medline]
AID - S0304-3940(12)00292-3 [pii]
AID - 10.1016/j.neulet.2012.02.066 [doi]
PST - ppublish
SO  - Neurosci Lett. 2012 Apr 11;514(1):96-101. doi: 10.1016/j.neulet.2012.02.066. Epub 
      2012 Mar 3.