PMID- 22405929
OWN - NLM
STAT- MEDLINE
DCOM- 20120619
LR  - 20120416
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 499
IP  - 1
DP  - 2012 May 10
TI  - Investigation on the transcription factors of porcine 3beta-hydroxysteroid 
      dehydrogenase and 17beta-hydroxysteroid dehydrogenase genes.
PG  - 186-90
LID - 10.1016/j.gene.2012.02.039 [doi]
AB  - The enzymes 3beta-hydroxysteroid dehydrogenase (3betaHSD) and 17beta-hydroxysteroid 
      dehydrogenase (17betaHSD) regulate the steroid metabolism in mammals. In this study, 
      we aimed to characterize the steroid related transcription factors at the 5' 
      flanking region of these two genes. A series of 5' deletions of approximately 1 
      kb of 5'-flanking region on both genes were fused to a pGL3 basic vector 
      containing firefly luciferase cDNA, and then transfected to human hepatocellular 
      liver carcinoma cell line (HepG2). Luciferase activity assay indicated the region 
      from -574 to -617 bp of the 3betaHSD1 promoter, and from -850 to -868 bp of 17betaHSD7 
      promoter induced the highest luciferase activity. A putative transcription 
      factor, i.e. the proline and acidic amino acid-rich basic leucine zipper 
      (PAR/bZIP) family of 3betaHSD1 gene, and three-amino acid loop extension (TALE) 
      homeodomain class of 17betaHSD7 were identified respectively by sequence homology. 
      Gel shift assay further confirmed the binding capacity of the putative elements 
      to nuclear extract. Our study gives new insights to the transcriptional 
      regulation of 3betaHSD1 and 17betaHSD7 and further hints to their involvement in 
      steroid metabolism.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Dong, Xinxing
AU  - Dong X
AD  - College of Animal Science and Technology, China Agricultural University, 100094 
      Beijing, China.
FAU - Bai, Ying
AU  - Bai Y
FAU - Xin, Yi
AU  - Xin Y
FAU - Xu, Qinqin
AU  - Xu Q
FAU - Chen, Gang
AU  - Chen G
FAU - Fang, Meiying
AU  - Fang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120228
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Transcription Factors)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/*genetics/metabolism
MH  - 3-Hydroxysteroid Dehydrogenases/*genetics/metabolism
MH  - Animals
MH  - Electrophoretic Mobility Shift Assay
MH  - Forecasting
MH  - *Gene Expression Regulation, Enzymologic/genetics
MH  - Hep G2 Cells
MH  - Humans
MH  - Swine/*genetics
MH  - Transcription Factors/*analysis/genetics/isolation & purification/physiology
MH  - Transfection
EDAT- 2012/03/13 06:00
MHDA- 2012/06/20 06:00
CRDT- 2012/03/13 06:00
PHST- 2011/11/13 00:00 [received]
PHST- 2012/01/31 00:00 [revised]
PHST- 2012/02/20 00:00 [accepted]
PHST- 2012/03/13 06:00 [entrez]
PHST- 2012/03/13 06:00 [pubmed]
PHST- 2012/06/20 06:00 [medline]
AID - S0378-1119(12)00241-7 [pii]
AID - 10.1016/j.gene.2012.02.039 [doi]
PST - ppublish
SO  - Gene. 2012 May 10;499(1):186-90. doi: 10.1016/j.gene.2012.02.039. Epub 2012 Feb 
      28.