PMID- 22405932
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20191210
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 413
IP  - 13-14
DP  - 2012 Jul 11
TI  - Evaluation of saliva collection devices for the analysis of proteins.
PG  - 1066-70
LID - 10.1016/j.cca.2012.02.020 [doi]
AB  - BACKGROUND: Human saliva mirrors the body's health and can be collected 
      non-invasively, does not require specialized skills and is suitable for large 
      population based screening programs. The aims were twofold: to evaluate the 
      suitability of commercially available saliva collection devices for quantifying 
      proteins present in saliva and to provide levels for C-reactive protein (CRP), 
      myoglobin, and immunoglobin E (IgE) in saliva of healthy individuals as a 
      baseline for future studies. METHODS: Saliva was collected from healthy 
      volunteers (n=17, ages 18-33years). The following collection methods were 
      evaluated: drool; Salimetrics(R) Oral Swab (SOS); Salivette(R) Cotton and Synthetic 
      (Sarstedt) and Greiner Bio-One Saliva Collection System (GBO SCS(R)). We used 
      AlphaLISA(R) assays to measure CRP, IgE and myoglobin levels in human saliva. 
      RESULTS: Significant (p<0.05) differences in the salivary flow rates were 
      observed based on the method of collection, i.e. salivary flow rates were 
      significantly lower (p<0.05) in unstimulated saliva (i.e. drool and SOS), when 
      compared with mechanically stimulated methods (p<0.05) (Salivette(R) Cotton and 
      Synthetic) and acid stimulated method (p<0.05) (SCS(R)). Saliva collected using SOS 
      yielded significantly (p<0.05) lower concentrations of myoglobin and CRP, whilst, 
      saliva collected using the Salivette(R) Cotton and Synthetic swab yielded 
      significantly (p<0.05) lower myoglobin and IgE concentrations respectively. 
      CONCLUSIONS: The results demonstrated significantly relevant differences in 
      analyte levels based on the collection method. Significant differences in the 
      salivary flow rates were also observed depending on the saliva collection method. 
      The data provide preliminary baseline values for salivary CRP, myoglobin, and IgE 
      levels in healthy participants and based on the collection method.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Topkas, Eleni
AU  - Topkas E
AD  - The Australian Institute for Bioengineering and Nanotechnology, The University of 
      Queensland, Queensland, Australia.
FAU - Keith, Patricia
AU  - Keith P
FAU - Dimeski, Goce
AU  - Dimeski G
FAU - Cooper-White, Justin
AU  - Cooper-White J
FAU - Punyadeera, Chamindie
AU  - Punyadeera C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120303
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Myoglobin)
RN  - 0 (Salivary Proteins and Peptides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - C-Reactive Protein/analysis
MH  - Female
MH  - Humans
MH  - *Immunoassay
MH  - Immunoglobulin E/analysis
MH  - Male
MH  - Myoglobin/analysis
MH  - Saliva/*chemistry
MH  - Salivary Proteins and Peptides/*analysis
MH  - Specimen Handling/*instrumentation
MH  - Young Adult
EDAT- 2012/03/13 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/03/13 06:00
PHST- 2011/12/14 00:00 [received]
PHST- 2012/01/30 00:00 [revised]
PHST- 2012/02/20 00:00 [accepted]
PHST- 2012/03/13 06:00 [entrez]
PHST- 2012/03/13 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - S0009-8981(12)00102-7 [pii]
AID - 10.1016/j.cca.2012.02.020 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2012 Jul 11;413(13-14):1066-70. doi: 10.1016/j.cca.2012.02.020. 
      Epub 2012 Mar 3.