PMID- 22406346
OWN - NLM
STAT- MEDLINE
DCOM- 20130128
LR  - 20211021
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 158
IP  - 1-2
DP  - 2012 Jul 6
TI  - Effect of porcine circovirus type 2a or 2b on infection kinetics and 
      pathogenicity of two genetically divergent strains of porcine reproductive and 
      respiratory syndrome virus in the conventional pig model.
PG  - 69-81
LID - 10.1016/j.vetmic.2012.02.010 [doi]
AB  - To determine differences in infection kinetics of two temporally and genetically 
      different type 2 porcine reproductive and respiratory syndrome virus (PRRSV) 
      isolates in vivo with and without concurrent porcine circovirus (PCV) type 2a or 
      2b infection, 62 pigs were randomly assigned to one of seven groups: negative 
      controls (n=8); pigs coinfected with a 1992 PRRSV strain (VR-2385) and PCV2a 
      (CoI-92-2a; n=9), pigs coinfected with VR-2385 and PCV2b (CoI-92-2b; n=9), pigs 
      coinfected with a 2006 PRRSV strain (NC16845b) and PCV2a (CoI-06-2a; n=9), pigs 
      coinfected with NC16845b and PCV2b (CoI-06-2b; n=9), pigs infected with VR-2385 
      (n=9), and pigs infected with NC16845b (n=9). Blood samples were collected before 
      inoculation and at day post-inoculation (dpi) 3, 6, 9 and 12 and tested for the 
      presence of PRRSV antibody and RNA, PCV2 antibody and DNA, complete blood counts, 
      and interferon gamma (IFN-gamma) levels. Regardless of concurrent PCV2 infection, 
      VR-2385 initially replicated at higher levels and reached peak replication levels 
      at dpi 6. Pigs infected with VR-2385 had significantly higher amounts of viral 
      RNA in serum on both dpi 3 and dpi 6, compared to pigs infected with NC16845b. 
      The peak of NC16845b virus replication occurred between dpi 9 and dpi 12 and was 
      associated with a delayed anti-PRRSV antibody response in these pigs. PCV2 
      coinfection resulted in significantly more severe macroscopic and microscopic 
      lung lesions and a stronger anti-PRRSV IgG response compared to pigs infected 
      with PRRSV alone. This work further emphasizes in vivo replication differences 
      among PRRSV strains and the importance of coinfecting pathogens.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. 
      tanjaopr@iastate.edu
FAU - Gauger, Phillip C
AU  - Gauger PC
FAU - Faaberg, Kay S
AU  - Faaberg KS
FAU - Shen, Huigang
AU  - Shen H
FAU - Beach, Nathan M
AU  - Beach NM
FAU - Meng, Xiang-Jin
AU  - Meng XJ
FAU - Wang, Chong
AU  - Wang C
FAU - Halbur, Patrick G
AU  - Halbur PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120217
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/analysis/blood
MH  - Circoviridae Infections/immunology/pathology/virology
MH  - Circovirus/*physiology
MH  - Coinfection
MH  - Kinetics
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/genetics/*pathogenicity
MH  - RNA, Viral/blood
MH  - Sus scrofa
MH  - Swine
PMC - PMC7117418
EDAT- 2012/03/13 06:00
MHDA- 2013/01/29 06:00
PMCR- 2012/02/17
CRDT- 2012/03/13 06:00
PHST- 2011/12/12 00:00 [received]
PHST- 2012/02/03 00:00 [revised]
PHST- 2012/02/09 00:00 [accepted]
PHST- 2012/03/13 06:00 [entrez]
PHST- 2012/03/13 06:00 [pubmed]
PHST- 2013/01/29 06:00 [medline]
PHST- 2012/02/17 00:00 [pmc-release]
AID - S0378-1135(12)00120-4 [pii]
AID - 10.1016/j.vetmic.2012.02.010 [doi]
PST - ppublish
SO  - Vet Microbiol. 2012 Jul 6;158(1-2):69-81. doi: 10.1016/j.vetmic.2012.02.010. Epub 
      2012 Feb 17.