PMID- 22410807
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160121
LR  - 20211021
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 64
IP  - 5
DP  - 2012 Oct
TI  - Evaluating the biodegradability of Gelatin/Siloxane/Hydroxyapatite (GS-Hyd) 
      complex in vivo and its ability for adhesion and proliferation of rat bone marrow 
      mesenchymal stem cells.
PG  - 485-95
LID - 10.1007/s10616-012-9426-5 [doi]
AB  - Recent studies have shown that the use of biomaterials and new biodegradable 
      scaffolds for repair or regeneration of damaged tissues is of vital importance. 
      Scaffolds used in tissue engineering should be biodegradable materials with 
      three-dimensional structures which guide the growth and differentiation of the 
      cells. They also tune physical, chemical and biological properties for efficient 
      supplying of the cells to the selected tissues and have proper porosity along 
      with minimal toxic effects. In this manner, the study of these characteristics is 
      a giant stride towards scaffold design. In this study, 
      Gelatin/Siloxane/Hydroxyapatite (GS-Hyd) scaffold was synthesized and its 
      morphology, in vivo biodegradability, cytotoxic effects and ability for cell 
      adhesion were investigated using mesenchymal stem cells (MSCs). The cells were 
      treated with different volumes of the scaffold suspension for evaluation of its 
      cytotoxic effects. The MSCs were also seeded on scaffolds and cultured for 
      2 weeks to evaluate the ability of the scaffold in promoting of cell adhesion and 
      growth. To check the biodegradability of the scaffold in vivo, scaffolds were 
      placed in the rat body for 21 days in three different positions of thigh muscle, 
      testicle, and liver and they were analyzed by scanning electron microscopy (SEM) 
      and weight changes. According to the results of the viability of this study, no 
      cytotoxic effects of GS-Hyd scaffold was found on the cells and MSCs could adhere 
      on the scaffold with expanding their elongations and forming colonies. The rate 
      of degradation as assessed by weight loss was significant within each group along 
      with significant differences between different tissues at the same time point. 
      SEM micrographs also indicated the obvious morphological changes on the surface 
      of the particles and diameter of the pores through different stages of 
      implantation. The greatest amount of degradation happened to the scaffold 
      particles implanted into the muscle, followed by testicle and liver, 
      respectively.
FAU - Neshati, Zeinab
AU  - Neshati Z
AD  - Cell and Molecular Biology Research Group, Institute of Biotechnology, Ferdowsi 
      University of Mashhad, Mashhad, Iran.
FAU - Bahrami, Ahmad Reza
AU  - Bahrami AR
FAU - Eshtiagh-Hosseini, Hossein
AU  - Eshtiagh-Hosseini H
FAU - Matin, Maryam M
AU  - Matin MM
FAU - Housaindokht, Mohammad Reza
AU  - Housaindokht MR
FAU - Tabari, Taymaz
AU  - Tabari T
FAU - Edalatmanesh, Mohammad Amin
AU  - Edalatmanesh MA
LA  - eng
PT  - Journal Article
DEP - 20120313
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3432534
EDAT- 2012/03/14 06:00
MHDA- 2012/03/14 06:01
PMCR- 2013/10/01
CRDT- 2012/03/14 06:00
PHST- 2011/08/09 00:00 [received]
PHST- 2012/01/03 00:00 [accepted]
PHST- 2012/03/14 06:00 [entrez]
PHST- 2012/03/14 06:00 [pubmed]
PHST- 2012/03/14 06:01 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - 9426 [pii]
AID - 10.1007/s10616-012-9426-5 [doi]
PST - ppublish
SO  - Cytotechnology. 2012 Oct;64(5):485-95. doi: 10.1007/s10616-012-9426-5. Epub 2012 
      Mar 13.