PMID- 22412050
OWN - NLM
STAT- MEDLINE
DCOM- 20120717
LR  - 20181201
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 65
IP  - 6
DP  - 2012 Jun
TI  - Epidermal growth factor receptor mutations in malignant pleural and peritoneal 
      mesothelioma.
PG  - 522-7
LID - 10.1136/jclinpath-2011-200631 [doi]
AB  - BACKGROUND: Epidermal growth factor receptor (EGFR) gene mutation at the kinase 
      domain and EGFR gene amplification are reported to be predictors of the response 
      to EGFR tyrosine kinase inhibitors in lung cancer cases. In malignant 
      mesothelioma (MM), the role of EGFR is less clear. METHODS: Thirty-eight MM 
      specimens were submitted to EGFR mutation evaluation, and compared with the 
      results of immunohistochemical staining and fluorescence in situ hybridization 
      (FISH) analysis. DNA was extracted from paraffin blocks and PCR was performed to 
      amplify exon regions 18-21 of the EGFR gene. Direct sequencing of the purified 
      PCR products was performed. RESULTS: Five EGFR missense mutations were detected 
      in six of the 38 patients (16%); two of these mutations were novel, two were 
      originally detected in non-small cell lung carcinoma, and one resembled a 
      location previously noted for malignant peritoneal mesothelioma. CONCLUSION: As 
      far as the authors are aware there has been no report of the EGFR mutation of MM 
      in Japanese cases, but in this study EGFR missense mutations were detected in 
      some cases. EGFR mutation results were not related to immunohistochemical and 
      FISH analysis.
FAU - Enomoto, Yasunori
AU  - Enomoto Y
AD  - Department of Diagnostic Pathology, Nara Medical University School of Medicine, 
      Nara, Japan.
FAU - Kasai, Takahiko
AU  - Kasai T
FAU - Takeda, Maiko
AU  - Takeda M
FAU - Takano, Masato
AU  - Takano M
FAU - Morita, Kohei
AU  - Morita K
FAU - Kadota, Eiji
AU  - Kadota E
FAU - Iizuka, Norishige
AU  - Iizuka N
FAU - Maruyama, Hiroshi
AU  - Maruyama H
FAU - Haratake, Joji
AU  - Haratake J
FAU - Kojima, Yu
AU  - Kojima Y
FAU - Ikeda, Naoya
AU  - Ikeda N
FAU - Inatsugi, Naoki
AU  - Inatsugi N
FAU - Nonomura, Akitaka
AU  - Nonomura A
LA  - eng
PT  - Journal Article
DEP - 20120312
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - DNA Mutational Analysis
MH  - DNA, Neoplasm/analysis
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mesothelioma/diagnosis/*genetics
MH  - Middle Aged
MH  - *Mutation, Missense
MH  - Peritoneal Neoplasms/diagnosis/*genetics
MH  - Pleural Neoplasms/diagnosis/*genetics
EDAT- 2012/03/14 06:00
MHDA- 2012/07/18 06:00
CRDT- 2012/03/14 06:00
PHST- 2012/03/14 06:00 [entrez]
PHST- 2012/03/14 06:00 [pubmed]
PHST- 2012/07/18 06:00 [medline]
AID - jclinpath-2011-200631 [pii]
AID - 10.1136/jclinpath-2011-200631 [doi]
PST - ppublish
SO  - J Clin Pathol. 2012 Jun;65(6):522-7. doi: 10.1136/jclinpath-2011-200631. Epub 
      2012 Mar 12.