PMID- 22414694
OWN - NLM
STAT- MEDLINE
DCOM- 20120516
LR  - 20211203
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 420
IP  - 1
DP  - 2012 Mar 30
TI  - Rapamycin suppresses the recurrent excitatory circuits of dentate gyrus in a 
      mouse model of temporal lobe epilepsy.
PG  - 199-204
LID - 10.1016/j.bbrc.2012.02.143 [doi]
AB  - Recurrent excitatory circuits and abnormal recurrent excitatory inputs are 
      essential in epileptogenesis. Studies in temporal lobe epilepsy have shown that 
      mossy fiber sprouting, which represents synaptic reorganization, renders the 
      formation of abnormal recurrent excitatory circuits and inputs. The mammalian 
      target of rapamycin (mTOR) pathway has recently been proved important in mossy 
      fiber sprouting. In the present study, rapamycin, a mTOR inhibiter, was injected 
      into the mouse of temporal lobe epilepsy. Electrophysiological and histological 
      properties of the hippocampus were investigated by whole cell patch clamp, 
      extracellular recording and Timm staining. Following the development of epilepsy, 
      frequency of spontaneous excitatory postsynaptic currents (EPSCs) and amplitude 
      of antidromically evoked EPSCs in granule cells were remarkably increased, as 
      well as the epileptiform activity and mossy fiber sprouting were detected, which 
      indicated the formation of abnormal recurrent excitatory circuits. By the use of 
      rapamycin, frequency of spontaneous EPSCs, amplitude of antidromically evoked 
      EPSCs, the epileptiform activity and mossy fiber sprouting were all remarkably 
      suppressed. Our findings suggested an anti-epileptogenic role of rapamycin by 
      suppressing the recurrent excitatory circuits of dentate gyrus.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Tang, Haiyun
AU  - Tang H
AD  - Department of Neurology, Xiangya Hospital, Central South University, Changsha, 
      Hunan 410008, China.
FAU - Long, Hongyu
AU  - Long H
FAU - Zeng, Chang
AU  - Zeng C
FAU - Li, Yi
AU  - Li Y
FAU - Bi, Fangfang
AU  - Bi F
FAU - Wang, Jinhui
AU  - Wang J
FAU - Qian, Hao
AU  - Qian H
FAU - Xiao, Bo
AU  - Xiao B
LA  - eng
PT  - Journal Article
DEP - 20120303
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anticonvulsants)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Anticonvulsants/*pharmacology
MH  - Dentate Gyrus/*drug effects/physiopathology
MH  - Disease Models, Animal
MH  - Epilepsy, Temporal Lobe/*physiopathology
MH  - Excitatory Postsynaptic Potentials/*drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Fibers/drug effects/physiology
MH  - Sirolimus/*pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors
EDAT- 2012/03/15 06:00
MHDA- 2012/05/17 06:00
CRDT- 2012/03/15 06:00
PHST- 2012/02/22 00:00 [received]
PHST- 2012/02/27 00:00 [accepted]
PHST- 2012/03/15 06:00 [entrez]
PHST- 2012/03/15 06:00 [pubmed]
PHST- 2012/05/17 06:00 [medline]
AID - S0006-291X(12)00402-0 [pii]
AID - 10.1016/j.bbrc.2012.02.143 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Mar 30;420(1):199-204. doi: 
      10.1016/j.bbrc.2012.02.143. Epub 2012 Mar 3.