PMID- 22415057
OWN - NLM
STAT- MEDLINE
DCOM- 20120907
LR  - 20120518
IS  - 1940-2465 (Electronic)
IS  - 1066-8969 (Linking)
VI  - 20
IP  - 3
DP  - 2012 Jun
TI  - Use of fluorescence in situ hybridization to distinguish metastatic uveal from 
      cutaneous melanoma.
PG  - 246-51
LID - 10.1177/1066896912438589 [doi]
AB  - Metastatic lesions of malignant melanoma can on occasion be difficult to classify 
      with regard to the primary site of origin. Given the lack of specificity of light 
      microscopic features, ancillary studies are needed. In this study, the authors 
      explored the possibility of distinguishing metastatic tumors derived from uveal 
      primaries from those known to have originated from a cutaneous melanoma by 
      fluorescence in situ hybridization (FISH) using probes for chromosome 3, 8q24, 
      and 1p36. A total of 32 metastatic tumors were analyzed by FISH. Monosomy 3 was 
      detected in 9 out of 16 (56.3%) cases of metastatic uveal melanoma but was not 
      found in any of the 16 metastatic cutaneous melanomas (P < .001). With regard to 
      1p36, amplifications were found in 8 out of 16 (50%) cases of metastatic 
      cutaneous melanoma but not in any case of uveal melanoma (P < .05). 1p36 was 
      deleted in 3 cases of uveal and 1 case of cutaneous melanoma. Amplifications of 
      8q were found in 15 out of 16 (94%) cases of uveal melanoma metastases and in 12 
      out of 16 (75%) cases of cutaneous metastases. The findings suggest that FISH for 
      monosomy 3 is a useful adjunct tool in the differential diagnosis of metastatic 
      uveal versus cutaneous melanoma.
FAU - Fang, Yuqiang
AU  - Fang Y
AD  - Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY 
      10065, USA.
FAU - Wang, Xuan
AU  - Wang X
FAU - Dusza, Stephen
AU  - Dusza S
FAU - Jhanwar, Suresh
AU  - Jhanwar S
FAU - Abramson, David
AU  - Abramson D
FAU - Busam, Klaus J
AU  - Busam KJ
LA  - eng
PT  - Journal Article
DEP - 20120313
PL  - United States
TA  - Int J Surg Pathol
JT  - International journal of surgical pathology
JID - 9314927
SB  - IM
MH  - Adult
MH  - Aged
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics/*secondary
MH  - Middle Aged
MH  - Skin Neoplasms/*genetics/*secondary
MH  - Uveal Neoplasms/*genetics/*pathology
MH  - Young Adult
EDAT- 2012/03/15 06:00
MHDA- 2012/09/08 06:00
CRDT- 2012/03/15 06:00
PHST- 2012/03/15 06:00 [entrez]
PHST- 2012/03/15 06:00 [pubmed]
PHST- 2012/09/08 06:00 [medline]
AID - 1066896912438589 [pii]
AID - 10.1177/1066896912438589 [doi]
PST - ppublish
SO  - Int J Surg Pathol. 2012 Jun;20(3):246-51. doi: 10.1177/1066896912438589. Epub 
      2012 Mar 13.