PMID- 22415246
OWN - NLM
STAT- MEDLINE
DCOM- 20121108
LR  - 20211021
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 29
IP  - 5
DP  - 2012 May
TI  - Methods for comprehensive chromosome screening of oocytes and embryos: 
      capabilities, limitations, and evidence of validity.
PG  - 381-90
LID - 10.1007/s10815-012-9727-9 [doi]
AB  - Preimplantation aneuploidy screening of cleavage stage embryos using fluorescence 
      in situ hybridization (FISH) may no longer be considered the standard of care in 
      reproductive medicine. Over the last few years, there has been considerable 
      development of novel technologies for comprehensive chromosome screening (CCS) of 
      the human genome. Among the notable methodologies that have been incorporated are 
      whole genome amplification, metaphase and array based comparative genomic 
      hybridization, single nucleotide polymorphism microarrays, and quantitative 
      real-time PCR. As these methods become more integral to treating patients with 
      infertility, it is critical that clinicians and scientists obtain a better 
      understanding of their capabilities and limitations. This article will focus on 
      reviewing these technologies and the evidence of their validity.
FAU - Treff, Nathan R
AU  - Treff NR
AD  - Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA. 
      ntreff@rmanj.com
FAU - Scott, Richard T Jr
AU  - Scott RT Jr
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20120314
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Comparative Genomic Hybridization/methods/standards
MH  - Female
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis/methods/standards
MH  - Oocytes/*physiology
MH  - Preimplantation Diagnosis/*methods/standards
MH  - Reproducibility of Results
PMC - PMC3348286
EDAT- 2012/03/15 06:00
MHDA- 2012/11/09 06:00
PMCR- 2012/03/14
CRDT- 2012/03/15 06:00
PHST- 2011/12/12 00:00 [received]
PHST- 2012/02/10 00:00 [accepted]
PHST- 2012/03/15 06:00 [entrez]
PHST- 2012/03/15 06:00 [pubmed]
PHST- 2012/11/09 06:00 [medline]
PHST- 2012/03/14 00:00 [pmc-release]
AID - 9727 [pii]
AID - 10.1007/s10815-012-9727-9 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2012 May;29(5):381-90. doi: 10.1007/s10815-012-9727-9. 
      Epub 2012 Mar 14.