PMID- 22415590
OWN - NLM
STAT- MEDLINE
DCOM- 20130318
LR  - 20211021
IS  - 1432-1262 (Electronic)
IS  - 0179-1958 (Print)
IS  - 0179-1958 (Linking)
VI  - 27
IP  - 11
DP  - 2012 Nov
TI  - The effect of inositol hexaphosphate on the expression of selected 
      metalloproteinases and their tissue inhibitors in IL-1beta-stimulated colon cancer 
      cells.
PG  - 1419-28
LID - 10.1007/s00384-012-1445-3 [doi]
AB  - INTRODUCTION: Matrix metalloproteinases (MMPs) have repeatedly been shown to play 
      a very active role in extracellular matrix degradation associated with tumor 
      invasion and metastasis. Tissue inhibitors of MMPs (TIMPs) are well-known for 
      their ability to inhibit MMP activity thereby inhibiting malignant progression. 
      Inositol hexaphosphate (IP6 phytic acid) has been recognized to have both 
      preventive and therapeutic effects against various cancers including that of 
      colon. In in vitro studies, IP6 has been demonstrated to inhibit cancer cell 
      adhesion and migration. In the present study, the effect of IP6 on the expression 
      of MMP and TIMP genes was evaluated in unstimulated and IL-1beta-stimulated colon 
      cancer cell line Caco-2. MATERIALS AND METHODS: Real-time QRT-PCR was used to 
      validate the transcription level of selected MMP and TIMP genes in Caco-2 cells 
      after treatment with 1 ng/ml of IL-1beta, 2.5 mM of IP6, and both for 6, 12, and 24 
      h. RESULTS: Stimulation of cells with IL-1beta only resulted in an overexpression of 
      MMP and their TIMP mRNAs. A significant decrease in MMP-13, MMP-3, MMP-2, and 
      TIMP-1 basal expression was achieved by IP6. IP6 was also an efficient 
      downregulator of MMP-1, MMP-9, and TIMP-2 genes transcription stimulated by IL-1beta 
      in 6 h lasting culture. After 12 h, IL-1beta-induced MMP-2 mRNA expression was 
      significantly reduced by IP6. CONCLUSION: Proinflammatory cytokine IL-1beta 
      upregulates MMP and TIMP mRNAs expression in colon cancer epithelial cells 
      Caco-2. IP6 (2.5 mM) influences constitutive expression of both MMP and TIMP 
      genes and downregulates IL-1beta stimulated transcription of some of these genes. 
      IP6 exerts its anti-metastatic activity through modulation of MMP and TIMP genes 
      expression to prevent cancer cell migration and invasion.
FAU - Kapral, Malgorzata
AU  - Kapral M
AD  - Department of Biochemistry, Medical University of Silesia, 41-200 Sosnowiec, 
      Narcyzow 1, Poland. mkapral@sum.edu.pl
FAU - Wawszczyk, Joanna
AU  - Wawszczyk J
FAU - Jurzak, Magdalena
AU  - Jurzak M
FAU - Hollek, Andrzej
AU  - Hollek A
FAU - Weglarz, Ludmila
AU  - Weglarz L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120315
PL  - Germany
TA  - Int J Colorectal Dis
JT  - International journal of colorectal disease
JID - 8607899
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 7IGF0S7R8I (Phytic Acid)
RN  - EC 3.4.- (Metalloproteases)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Caco-2 Cells
MH  - Collagenases/genetics/metabolism
MH  - Colonic Neoplasms/*enzymology/*genetics/pathology
MH  - Gelatinases/genetics/metabolism
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Interleukin-1beta/*pharmacology
MH  - Matrix Metalloproteinase 3/genetics/metabolism
MH  - Metalloproteases/*genetics/metabolism
MH  - Models, Biological
MH  - Neoplasm Metastasis
MH  - Phytic Acid/*pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinases/*genetics/metabolism
PMC - PMC3474917
EDAT- 2012/03/15 06:00
MHDA- 2013/03/19 06:00
PMCR- 2012/03/15
CRDT- 2012/03/15 06:00
PHST- 2012/02/29 00:00 [accepted]
PHST- 2012/03/15 06:00 [entrez]
PHST- 2012/03/15 06:00 [pubmed]
PHST- 2013/03/19 06:00 [medline]
PHST- 2012/03/15 00:00 [pmc-release]
AID - 1445 [pii]
AID - 10.1007/s00384-012-1445-3 [doi]
PST - ppublish
SO  - Int J Colorectal Dis. 2012 Nov;27(11):1419-28. doi: 10.1007/s00384-012-1445-3. 
      Epub 2012 Mar 15.