PMID- 22415667
OWN - NLM
STAT- MEDLINE
DCOM- 20120731
LR  - 20120314
IS  - 1536-4828 (Electronic)
IS  - 0885-3177 (Linking)
VI  - 41
IP  - 3
DP  - 2012 Apr
TI  - Pancreatic stellate cells do not exhibit features of antigen-presenting cells.
PG  - 422-7
LID - 10.1097/MPA.0b013e31822e673b [doi]
AB  - OBJECTIVES: Major histocompatibility complex (MHC) class II molecules are 
      expressed on professional antigen-presenting cells (APCs), and pancreatic 
      stellate cells (PSCs) have endocytic and phagocytic functions and play a role in 
      the immune responses of the pancreas. The aim of the present study was to 
      investigate whether PSCs exhibit features of APCs. METHODS: Rat and human PSCs 
      were cultured with interferon-gamma (IFN-gamma) or an exogenous antigen, ovalbumin (OVA), 
      and they were analyzed for expression of MHC II molecules by flow cytometry and 
      reverse transcription-polymerase chain reaction. RESULTS: The cells simulated 
      with IFN-gamma expressed very little or no MHC class II molecules or human leukocyte 
      antigen (HLA)-DR at the transcriptional level. Stimulation with IFN-gamma failed to 
      induce expression of MHC class II molecules and HLA-DR molecules according to the 
      results of flow cytometry. Dual-color flow cytometric analysis showed that 
      approximately 95% of the PSCs took up OVA; however, none of the cells that took 
      up OVA expressed MHC class II molecules or HLA-DR molecules. CONCLUSIONS: 
      Pancreatic stellate cells do not seem to be responsible for the MHC class 
      II-dependent pathway of antigen presentation, suggesting that PSCs do not play a 
      role in adaptive immunity as APCs.
FAU - Shimizu, Kyoko
AU  - Shimizu K
AD  - Department of Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan. 
      kyoko@ige.twmu.ac.jp
FAU - Hashimoto, Kahoko
AU  - Hashimoto K
FAU - Tahara, Junko
AU  - Tahara J
FAU - Imaeda, Hirotsugu
AU  - Imaeda H
FAU - Andoh, Akira
AU  - Andoh A
FAU - Shiratori, Keiko
AU  - Shiratori K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigen-Presenting Cells/*immunology
MH  - Cells, Cultured
MH  - Endocytosis
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - HLA-DR Antigens/genetics/metabolism
MH  - Histocompatibility Antigens Class II/genetics/metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Male
MH  - Ovalbumin/metabolism
MH  - Pancreatic Stellate Cells/*immunology
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic
EDAT- 2012/03/15 06:00
MHDA- 2012/08/01 06:00
CRDT- 2012/03/15 06:00
PHST- 2012/03/15 06:00 [entrez]
PHST- 2012/03/15 06:00 [pubmed]
PHST- 2012/08/01 06:00 [medline]
AID - 00006676-201204000-00010 [pii]
AID - 10.1097/MPA.0b013e31822e673b [doi]
PST - ppublish
SO  - Pancreas. 2012 Apr;41(3):422-7. doi: 10.1097/MPA.0b013e31822e673b.