PMID- 22416221
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20211021
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Print)
IS  - 1226-4512 (Linking)
VI  - 16
IP  - 1
DP  - 2012 Feb
TI  - Requirement of Pretone by Thromboxane A(2) for Hypoxic Pulmonary Vasoconstriction 
      in Precision-cut Lung Slices of Rat.
PG  - 59-64
LID - 10.4196/kjpp.2012.16.1.59 [doi]
AB  - Hypoxic pulmonary vasoconstriction (HPV) is physiologically important response 
      for preventing mismatching between ventilation and perfusion in lungs. The HPV of 
      isolated pulmonary arteries (HPV-PA) usually require a partial pretone by 
      thromboxane agonist (U46619). Because the HPV of ventilated/perfused lungs 
      (HPV-lung) can be triggered without pretone conditioning, we suspected that a 
      putative tissue factor might be responsible for the pretone of HPV. Here we 
      investigated whether HPV can be also observed in precision-cut lung slices (PCLS) 
      from rats. The HPV in PCLS also required partial contraction by U46619. In 
      addition, K(+) channel blockers (4AP and TEA) required U46619-pretone to induce 
      significant contraction of PA in PCLS. In contrast, the airways in PCLS showed 
      reversible contraction in response to the K(+) channel blockers without pretone 
      conditioning. Also, the airways showed no hypoxic constriction but a relaxation 
      under the partial pretone by U46619. The airways in PCLS showed reliable, 
      concentration-dependent contraction by metacholine (EC(50), ~210 nM). In summary, 
      the HPV in PCLS is more similar to isolated PA than V/P lungs. The 
      metacholine-induced constriction of bronchioles suggested that the PLCS might be 
      also useful for studying airway physiology in situ.
FAU - Park, Su Jung
AU  - Park SJ
AD  - Department of Physiology, Seoul National University College of Medicine, Seoul 
      110-799, Korea.
FAU - Yoo, Hae Young
AU  - Yoo HY
FAU - Kim, Hye Jin
AU  - Kim HJ
FAU - Kim, Jin Kyoung
AU  - Kim JK
FAU - Zhang, Yin-Hua
AU  - Zhang YH
FAU - Kim, Sung Joon
AU  - Kim SJ
LA  - eng
PT  - Journal Article
DEP - 20120228
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC3298827
OTO - NOTNLM
OT  - Airway smooth muscle
OT  - Hypoxic pulmonary vasoconstriction
OT  - Lung slice
OT  - Thromboxane A2
EDAT- 2012/03/15 06:00
MHDA- 2012/03/15 06:01
PMCR- 2012/02/01
CRDT- 2012/03/15 06:00
PHST- 2012/01/10 00:00 [received]
PHST- 2012/01/22 00:00 [revised]
PHST- 2012/01/25 00:00 [accepted]
PHST- 2012/03/15 06:00 [entrez]
PHST- 2012/03/15 06:00 [pubmed]
PHST- 2012/03/15 06:01 [medline]
PHST- 2012/02/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2012.16.1.59 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2012 Feb;16(1):59-64. doi: 10.4196/kjpp.2012.16.1.59. 
      Epub 2012 Feb 28.