PMID- 22421568
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20200930
IS  - 1523-7834 (Print)
IS  - 1523-7834 (Linking)
VI  - 38
IP  - 1
DP  - 2012
TI  - Patterns of BCR/ABL Gene Rearrangements in Chronic Myeloid Leukemia with Complex 
      t(9;22) Using Fluorescence In Situ Hybridization (FISH).
PG  - 5-7
AB  - Chronic myeloid leukemia (CML) is characterized by the reciprocal translocation 
      t(9;22)(q34;q11.2) which fuses the ABL1 oncogene on chromosome 9 with the BCR 
      gene on chromosome 22. It is the BCR/ABL protein that drives the neoplasm and the 
      ABL/BCR is not necessary for the disease. In the majority of CML cases, the 
      BCR/ABL fusion gene is cytogenetically recognizable as a small derivative 
      chromosome 22(der 22), which is known as the Philadelphia (Ph) chromosome. 
      However, approximately 2-10% of patients with CML involve cryptic or complex 
      variant translocations with deletions on the der(9) and/or der(22) occuring in 
      roughly 10-15% of CML cases. Fluorescence in situ hybridization (FISH) analysis 
      can help identify deletions and complex or cryptic rearrangements. Various 
      BCR/ABL FISH probes are available, which include dual color single fusion, dual 
      color extra signal (ES), dual color dual fusion and tri color dual fusion probes. 
      To test the utility of these probes, six patients diagnosed with CML carrying 
      different complex variant Ph translocations were studied by G-banding and FISH 
      analysis using the BCR/ABL ES, BCR/ABL dual color dual fusion, and BCR/ABL 
      tricolor probes. There are differences among the probes in their ability to 
      detect variant rearrangements, with or without accompanying chromoso me 9 and/or 
      22 deletions, and low level disease.
FAU - Esan, Olukemi A
AU  - Esan OA
AD  - Department of Pathology, West Virginia University, Morgantown, WV.
FAU - Senft, Jamie R
AU  - Senft JR
FAU - Wenger, Sharon L
AU  - Wenger SL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Assoc Genet Technol
JT  - Journal of the Association of Genetic Technologists
JID - 9807282
EDAT- 2012/03/17 06:00
MHDA- 2012/03/17 06:01
CRDT- 2012/03/17 06:00
PHST- 2012/03/17 06:00 [entrez]
PHST- 2012/03/17 06:00 [pubmed]
PHST- 2012/03/17 06:01 [medline]
PST - ppublish
SO  - J Assoc Genet Technol. 2012;38(1):5-7.