PMID- 22423651
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20211021
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 43
IP  - 1
DP  - 2012 Mar 16
TI  - Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV 
      infection.
PG  - 19
LID - 10.1186/1297-9716-43-19 [doi]
AB  - The presence of foreign cells within the tissue/circulation of an individual is 
      described as microchimerism. The main purpose of the present investigation was to 
      study if microchimerism occurs in healthy sows/fetuses and if porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection influences this 
      phenomenon. Six dams were inoculated intranasally with PRRSV and three 
      non-inoculated dams served as controls. Male DNA was detected in female fetal 
      sera of all dams via PCR. Male DNA was also detected in the maternal circulation. 
      Sex-typing FISH showed the presence of male cells in the female fetal organs and 
      vice versa. PRRSV infection did not influence microchimerism, but might misuse 
      maternal and sibling microchimeric cells to enter fetuses.
FAU - Karniychuk, Uladzimir U
AU  - Karniychuk UU
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Ghent, 
      Belgium. uladzimir.karniychuk@UGent.be.
FAU - Van Breedam, Wander
AU  - Van Breedam W
FAU - Van Roy, Nadine
AU  - Van Roy N
FAU - Rogel-Gaillard, Claire
AU  - Rogel-Gaillard C
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120316
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Chimerism/*veterinary
MH  - DNA/*blood
MH  - Female
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Male
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*physiopathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Pregnancy
MH  - Swine/*genetics
PMC - PMC3368719
EDAT- 2012/03/20 06:00
MHDA- 2014/06/03 06:00
PMCR- 2012/01/01
CRDT- 2012/03/20 06:00
PHST- 2011/07/15 00:00 [received]
PHST- 2012/03/16 00:00 [accepted]
PHST- 2012/03/20 06:00 [entrez]
PHST- 2012/03/20 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 1297-9716-43-19 [pii]
AID - 10.1186/1297-9716-43-19 [doi]
PST - epublish
SO  - Vet Res. 2012 Mar 16;43(1):19. doi: 10.1186/1297-9716-43-19.