PMID- 22426356
OWN - NLM
STAT- MEDLINE
DCOM- 20121009
LR  - 20141120
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 50
IP  - 6
DP  - 2012 Jun
TI  - Uncaria tomentosa extracts protect human erythrocyte catalase against damage 
      induced by 2,4-D-Na and its metabolites.
PG  - 2123-7
LID - 10.1016/j.fct.2012.02.099 [doi]
AB  - The effect of ethanolic and aqueous extracts from leaves and bark of Uncaria 
      tomentosa was studied, with particular attention to catalase activity (CAT - EC. 
      1.11.1.6). We observed that all tested extracts, at a concentration of 250 mug/mL 
      were not toxic to erythrocyte catalase because they did not decreased its 
      activity. Additionally, we investigated the protective effect of extracts on 
      changes in CAT activity in the erythrocytes incubated with sodium salt of 
      2,4-dichlorophenoxyacetic acid (2,4-D-Na) and its metabolites i.e., 
      2,4-dichlorophenol (2,4-DCP) and catechol. Previous investigations showed that 
      these chemicals decreased activity of erythrocyte catalase (Bukowska et al., 
      2000; Bukowska and Kowalska, 2004). The erythrocytes were divided into two 
      portions. The first portion was incubated for 1 and 5h at 37 degrees C with 2,4-D-Na, 
      2,4-DCP and catechol, and second portion was preincubated with extracts for 10 
      min and then incubated with xenobiotics for 1 and 5h. CAT activity was measured 
      in the first and second portion of the erythrocytes. We found a protective effect 
      of the extracts from U. tomentosa on the activity of catalase incubated with 
      xenobiotics studied. Probably, phenolic compounds contained in U. tomentosa 
      scavenged free radicals, and therefore protected active center (containing -SH 
      groups) of catalase.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Bukowska, Bozena
AU  - Bukowska B
AD  - Department of Environmental Pollution Biophysics, Pomorska 141/143 St., 90-237 
      Lodz, Poland. bukow@biol.uni.lodz.pl
FAU - Bors, Milena
AU  - Bors M
FAU - Gulewicz, Krzysztof
AU  - Gulewicz K
FAU - Koter-Michalak, Maria
AU  - Koter-Michalak M
LA  - eng
PT  - Journal Article
DEP - 20120309
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Antioxidants)
RN  - 0 (Catechols)
RN  - 0 (Chlorophenols)
RN  - 0 (Herbicides)
RN  - 0 (Plant Extracts)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
RN  - EC 1.11.1.6 (Catalase)
RN  - LF3AJ089DQ (catechol)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/*antagonists & inhibitors/*toxicity
MH  - Antioxidants/metabolism
MH  - Cat's Claw/*chemistry
MH  - Catalase/*antagonists & inhibitors/blood/*metabolism
MH  - Catechols/antagonists & inhibitors/toxicity
MH  - Chlorophenols/antagonists & inhibitors/toxicity
MH  - Erythrocytes/drug effects/*enzymology
MH  - Herbicides/*antagonists & inhibitors/*toxicity
MH  - Humans
MH  - In Vitro Techniques
MH  - Plant Bark/chemistry
MH  - Plant Extracts/pharmacology
MH  - Plant Leaves
EDAT- 2012/03/20 06:00
MHDA- 2012/10/10 06:00
CRDT- 2012/03/20 06:00
PHST- 2011/12/20 00:00 [received]
PHST- 2012/02/08 00:00 [revised]
PHST- 2012/02/28 00:00 [accepted]
PHST- 2012/03/20 06:00 [entrez]
PHST- 2012/03/20 06:00 [pubmed]
PHST- 2012/10/10 06:00 [medline]
AID - S0278-6915(12)00183-4 [pii]
AID - 10.1016/j.fct.2012.02.099 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2012 Jun;50(6):2123-7. doi: 10.1016/j.fct.2012.02.099. Epub 
      2012 Mar 9.