PMID- 22427154
OWN - NLM
STAT- MEDLINE
DCOM- 20130130
LR  - 20211021
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 35
IP  - 4
DP  - 2012 Aug
TI  - Inhibition of titanium particle-induced inflammation by the proteasome inhibitor 
      bortezomib in murine macrophage-like RAW 264.7 cells.
PG  - 1411-8
LID - 10.1007/s10753-012-9454-5 [doi]
AB  - Wear particle-induced inflammatory osteolysis is the major cause of aseptic 
      loosening after total joint replacement. The predominant cell type within 
      periprosthetic tissues is macrophages. We investigate the anti-inflammatory 
      effects of the proteasome inhibitor bortezomib (Bzb) on murine macrophage-like 
      RAW 264.7 cells stimulated with titanium (Ti) particles. RAW 264.7 cells were 
      cultured with 1 nM Bzb and 0.1 mg/ml Ti particles for 48 h; cells without Ti and 
      Bzb or without Bzb were used as negative and loading controls. Results showed 
      that the expression of inflammatory cytokines (TNF-alpha, IL-1beta, IL-6, and IL-10), 
      chemokines [monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory 
      protein-1 alpha (MIP-1alpha)], and inflammatory enzymes [inducible nitric oxide 
      synthase (iNOS), cyclooxygenase-2 (COX-2)] increased in RAW 264.7 cells cultured 
      with Ti. Bzb treatment significantly reduced the expression of TNF-alpha, IL-1beta, 
      IL-6, MCP-1, MIP-1alpha, iNOS, and COX-2 and induced the expression of IL-10 in a 
      time-dependent manner. These results suggest that Bzb inhibits Ti-induced 
      inflammation in macrophages, and provide a promising therapeutic target for 
      treating or preventing aseptic loosening.
FAU - Mao, Xin
AU  - Mao X
AD  - Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai 
      Jiaotong University School of Medicine, 600 Yishan Road, Shanghai, 200233, China.
FAU - Pan, Xiaoyun
AU  - Pan X
FAU - Peng, Xiaochun
AU  - Peng X
FAU - Cheng, Tao
AU  - Cheng T
FAU - Zhang, Xianlong
AU  - Zhang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Boronic Acids)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Proteasome Inhibitors)
RN  - 0 (Pyrazines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 69G8BD63PP (Bortezomib)
RN  - D1JT611TNE (Titanium)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
MH  - Boronic Acids/*pharmacology
MH  - Bortezomib
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Chemokine CCL2/biosynthesis
MH  - Cyclooxygenase 2/biosynthesis
MH  - Inflammation/chemically induced/*drug therapy
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-1beta/biosynthesis/drug effects
MH  - Interleukin-6/biosynthesis
MH  - Macrophage Inflammatory Proteins/biosynthesis
MH  - Macrophages/*drug effects/immunology/metabolism
MH  - Mice
MH  - Nitric Oxide Synthase Type II/biosynthesis
MH  - Proteasome Inhibitors/*pharmacology
MH  - Pyrazines/*pharmacology
MH  - Titanium/*pharmacology
MH  - Tumor Necrosis Factor-alpha/biosynthesis/drug effects
EDAT- 2012/03/20 06:00
MHDA- 2013/01/31 06:00
CRDT- 2012/03/20 06:00
PHST- 2012/03/20 06:00 [entrez]
PHST- 2012/03/20 06:00 [pubmed]
PHST- 2013/01/31 06:00 [medline]
AID - 10.1007/s10753-012-9454-5 [doi]
PST - ppublish
SO  - Inflammation. 2012 Aug;35(4):1411-8. doi: 10.1007/s10753-012-9454-5.