PMID- 22427501
OWN - NLM
STAT- MEDLINE
DCOM- 20120815
LR  - 20240321
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 78
IP  - 10
DP  - 2012 May
TI  - Quantitative fluorescence in situ hybridization analysis of microbial consortia 
      from a biogenic gas field in Alaska's Cook Inlet basin.
PG  - 3599-605
LID - 10.1128/AEM.07122-11 [doi]
AB  - Filter-collected production water samples from a methane-rich gas field in the 
      Cook Inlet basin of Alaska were investigated using whole-cell rRNA-targeted 
      fluorescence in situ hybridization (FISH) and 16S rRNA tag pyrosequencing. Both 
      techniques were consistent in determining the microbial community composition, 
      including the archaeal or bacterial dominance of samples. The archaeal community 
      is dominated by the obligate methylotrophic methanogen genus Methanolobus as well 
      as the nutritional generalist methanogen genus Methanosarcina, which is capable 
      of utilizing acetate, CO(2), and methyl-bearing compounds. The most-abundant 
      bacterial groups are Firmicutes, notably of the Acetobacterium genus, and 
      Cytophaga-Flexibacter-Bacteroides species (CFBs) affiliated with the order 
      Bacteroidales. We observed spatial variation among samples in both the percentage 
      of members of Archaea compared to that of members of Bacteria and the dominant 
      members of the bacterial community, differences which could not be explained with 
      the available geochemical data. Based upon the microbial community composition 
      and the isotopic signature of methane associated with the Cook Inlet basin site, 
      we propose a simplified reaction network beginning with the breakdown of coal 
      macromolecules, followed by fermentation and methylotrophic and acetoclastic 
      methane production.
FAU - Dawson, Katherine S
AU  - Dawson KS
AD  - Pennsylvania State University, University Park, Pennsylvania, USA. 
      katsdawson@psu.edu
FAU - Strapoc, Dariusz
AU  - Strapoc D
FAU - Huizinga, Brad
AU  - Huizinga B
FAU - Lidstrom, Ulrika
AU  - Lidstrom U
FAU - Ashby, Matt
AU  - Ashby M
FAU - Macalady, Jennifer L
AU  - Macalady JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120316
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Acetates)
RN  - 142M471B3J (Carbon Dioxide)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Acetates/metabolism
MH  - Alaska
MH  - Archaea/*classification/genetics
MH  - Bacteria/*classification/genetics
MH  - *Biota
MH  - Carbon Dioxide/metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Methane/metabolism
MH  - *Microbial Consortia
MH  - Oil and Gas Fields/*microbiology
MH  - Sequence Analysis, DNA
MH  - *Water Microbiology
PMC - PMC3346356
EDAT- 2012/03/20 06:00
MHDA- 2012/08/16 06:00
PMCR- 2012/11/01
CRDT- 2012/03/20 06:00
PHST- 2012/03/20 06:00 [entrez]
PHST- 2012/03/20 06:00 [pubmed]
PHST- 2012/08/16 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - AEM.07122-11 [pii]
AID - 07122-11 [pii]
AID - 10.1128/AEM.07122-11 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2012 May;78(10):3599-605. doi: 10.1128/AEM.07122-11. Epub 
      2012 Mar 16.