PMID- 22427853
OWN - NLM
STAT- MEDLINE
DCOM- 20120820
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 3
DP  - 2012
TI  - The differential proliferative ability of satellite cells in Lantang and Landrace 
      pigs.
PG  - e32537
LID - 10.1371/journal.pone.0032537 [doi]
LID - e32537
AB  - Here, for the first time, we evaluate the hypothesis that the proliferative 
      abilities of satellite cells (SCs) isolated from Lantang (indigenous Chinese 
      pigs) and Landrace pigs, which differ in muscle characteristics, are different. 
      SCs were isolated from the longissimus dorsi muscle of neonatal Lantang and 
      Landrace pigs. Proliferative ability was estimated by the count and proliferative 
      activity of viable cells using a hemocytometer and MTT assay at different time 
      points after seeding, respectively. Cell cycle information was detected by flow 
      cytometry. Results showed that there was a greater (P<0.05) number of SCs in 
      Lantang pigs compared with Landrace pigs after 72 h of culture. The percentage of 
      cell population in S phase and G(2)/M phases in Lantang pigs were higher 
      (P<0.05), while in G(0)/G(1) phase was lower (P<0.05) in comparison with the 
      Landrace pigs. The mRNA abundances of MyoD, Myf5, myogenin and Pax7 in SCs from 
      Lantang pigs were higher (P<0.05), while those of myostatin, Smad3 and genes in 
      the mammalian target of rapamycin (mTOR) pathway (with the exception of 4EBP1) 
      were lower (P<0.05) than the Landrace pigs. Protein levels of MyoD, myogenin, 
      myostatin, S6K, phosphorylated mTOR and phosphorylated eIF4E were consistent with 
      the corresponding mRNA abundance. Collectively, these findings suggested that SCs 
      in the two breeds present different proliferative abilities, and the 
      proliferative potential of SCs in Lantang pigs is higher than in Landrace pigs.
FAU - Wang, Xiu-qi
AU  - Wang XQ
AD  - College of Animal Science, South China Agricultural University, Guangzhou, 
      Guangdong Province, China. xqwang@scau.edu.cn
FAU - Yang, Wei-jun
AU  - Yang WJ
FAU - Yang, Zhou
AU  - Yang Z
FAU - Shu, Gang
AU  - Shu G
FAU - Wang, Song-bo
AU  - Wang SB
FAU - Jiang, Qing-yan
AU  - Jiang QY
FAU - Yuan, Li
AU  - Yuan L
FAU - Wu, Tong-shan
AU  - Wu TS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120313
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (MyoD Protein)
RN  - 0 (Myogenic Regulatory Factor 5)
RN  - 0 (Myogenin)
RN  - 0 (Myostatin)
RN  - 0 (PAX7 Transcription Factor)
RN  - 0 (Smad3 Protein)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Cycle/physiology
MH  - *Cell Proliferation
MH  - Flow Cytometry
MH  - Muscle, Skeletal/*cytology
MH  - MyoD Protein/metabolism
MH  - Myogenic Regulatory Factor 5/metabolism
MH  - Myogenin/metabolism
MH  - Myostatin/metabolism
MH  - PAX7 Transcription Factor/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Satellite Cells, Skeletal Muscle/*physiology
MH  - Smad3 Protein/metabolism
MH  - Species Specificity
MH  - Sus scrofa/*physiology
MH  - Tetrazolium Salts
MH  - Thiazoles
PMC - PMC3302802
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/03/20 06:00
MHDA- 2012/08/21 06:00
PMCR- 2012/03/13
CRDT- 2012/03/20 06:00
PHST- 2011/07/31 00:00 [received]
PHST- 2012/01/30 00:00 [accepted]
PHST- 2012/03/20 06:00 [entrez]
PHST- 2012/03/20 06:00 [pubmed]
PHST- 2012/08/21 06:00 [medline]
PHST- 2012/03/13 00:00 [pmc-release]
AID - PONE-D-11-15008 [pii]
AID - 10.1371/journal.pone.0032537 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(3):e32537. doi: 10.1371/journal.pone.0032537. Epub 2012 Mar 13.