PMID- 22428781
OWN - NLM
STAT- MEDLINE
DCOM- 20130521
LR  - 20121211
IS  - 1607-8888 (Electronic)
IS  - 1025-3890 (Linking)
VI  - 16
IP  - 1
DP  - 2013 Jan
TI  - Chronic psychological stress induces vascular inflammation in rabbits.
PG  - 87-98
LID - 10.3109/10253890.2012.676696 [doi]
AB  - Psychological stress is associated with a systemic inflammatory response. It is 
      unclear, however, whether psychological stress contributes to vascular 
      inflammation. Here, we examined the effects of unpredictable chronic mild stress 
      (UCMS) on vascular inflammation in rabbits. One hundred rabbits were randomly 
      divided into control and stress groups. UCMS was induced by a set of defined 
      adverse conditions applied in a shuffled order for 4, 8, 12, or 16 weeks, and 
      rabbits were killed 24 h after the end of the UCMS protocol. Expression of 
      different inflammatory molecules was analyzed by real-time polymerase chain 
      reaction, immunohistochemistry, or enzyme-linked immunosorbent assay. UCMS 
      resulted in depression-like behaviors, decreased body weight gain, and 
      hypertension with no significant effects on serum lipids. Aortic mRNA and protein 
      expression for tumor necrosis factor-alpha (TNF-alpha), C-reactive protein (CRP), 
      monocyte chemoattractant protein-1 (MCP-1), macrophage migration inhibitory 
      factor, and expression of intercellular adhesion molecule-1 (ICAM-1) protein were 
      increased. UCMS increased circulating concentrations of corticosterone, TNF-alpha, 
      and CRP throughout. Moreover, stress downregulated the expression of endothelial 
      nitric oxide synthase. At 16 weeks of UCMS, macrophage infiltration and lipid 
      accumulation in the subendothelial space were detected in the aorta. In cultured 
      murine vascular smooth muscle cells, treatment with serum from stressed rabbits 
      significantly increased phosphorylation of p38 and c-Jun N-terminal kinase (JNK), 
      and upregulated expression of MCP-1 and ICAM-1 mRNAs, in which the effect was 
      blunted by a TNF-alpha neutralizing antibody or p38 and JNK inhibitors. Our results 
      indicate that chronic psychological stress induces vascular inflammation via 
      TNF-alpha and p38/JNK pathways, which may contribute to the development of 
      atherosclerosis.
FAU - Lu, Xiao Ting
AU  - Lu XT
AD  - Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese 
      Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Shandong 
      University, Jinan, Shandong Province, PR China.
FAU - Liu, Yun Fang
AU  - Liu YF
FAU - Zhao, Li
AU  - Zhao L
FAU - Li, Wen Jing
AU  - Li WJ
FAU - Yang, Rui Xue
AU  - Yang RX
FAU - Yan, Fang Fang
AU  - Yan FF
FAU - Zhao, Yu Xia
AU  - Zhao YX
FAU - Jiang, Fan
AU  - Jiang F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120425
PL  - England
TA  - Stress
JT  - Stress (Amsterdam, Netherlands)
JID - 9617529
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Blotting, Western
MH  - Body Weight/physiology
MH  - Cells, Cultured
MH  - Chronic Disease
MH  - Eating/physiology
MH  - Hemodynamics/physiology
MH  - Homeostasis/physiology
MH  - Hypothalamo-Hypophyseal System/physiology
MH  - Immunohistochemistry
MH  - Lipid Metabolism/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myocytes, Smooth Muscle/physiology
MH  - Noise/adverse effects
MH  - Periodicity
MH  - Pituitary-Adrenal System/physiology
MH  - Rabbits
MH  - Real-Time Polymerase Chain Reaction
MH  - Restraint, Physical
MH  - Stress, Psychological/*complications/*pathology/psychology
MH  - Vasculitis/*etiology/*pathology
EDAT- 2012/03/21 06:00
MHDA- 2013/05/23 06:00
CRDT- 2012/03/21 06:00
PHST- 2012/03/21 06:00 [entrez]
PHST- 2012/03/21 06:00 [pubmed]
PHST- 2013/05/23 06:00 [medline]
AID - 10.3109/10253890.2012.676696 [doi]
PST - ppublish
SO  - Stress. 2013 Jan;16(1):87-98. doi: 10.3109/10253890.2012.676696. Epub 2012 Apr 
      25.