PMID- 22429009
OWN - NLM
STAT- MEDLINE
DCOM- 20120926
LR  - 20181201
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 18
IP  - 11-12
DP  - 2012 Jun
TI  - Effect of enamel matrix derivative and of proline-rich synthetic peptides on the 
      differentiation of human mesenchymal stem cells toward the osteogenic lineage.
PG  - 1253-63
LID - 10.1089/ten.tea.2011.0404 [doi]
AB  - With the aim of discovering new molecules for induction of bone formation and 
      biomineralization, combination of bioinformatics and simulation methods were used 
      to design the structure of artificial peptides based on proline-rich domains of 
      enamel matrix proteins. In this study, the effect of such peptides on the 
      differentiation toward the osteogenic lineage of human umbilical cord mesenchymal 
      stem cells (hUCMSCs) was evaluated with or without osteogenic supplements 
      (hydrocortisone, beta-glycerol phosphate, and ascorbic acid) and compared to the 
      effect of the commercially available enamel matrix derivative (EMD). It was 
      hypothesized that the differentiation toward the osteogenic lineage of hUCMSCs 
      would be promoted by the treatment with the synthetic peptides when combined with 
      differentiation media, or it could even be directed exclusively by the synthetic 
      peptides. Osteoinductivity was assessed by cell proliferation, bone morphogenetic 
      protein-2 secretion, and gene expression of osteogenic markers after 1, 3, and 14 
      days of treatment. All peptides were safe with the dosages used, showing lower 
      cell toxicity. P2, P4, and P6 reduced cell proliferation with growing media by 
      10%-15%. Higher expression of early osteoblast markers was found after 3 days of 
      treatment with EMD in combination with osteogenic supplements, while after 14 
      days of treatment, cells treated by the different synthetic peptides in 
      combination with osteogenic supplements showed higher osteocalcin mRNA levels. We 
      can conclude that osteogenic differentiation of hUCMSCs is promoted by short-term 
      EMD treatment in combination with osteogenic supplements and by long-term 
      treatment by the synthetic peptides in combination with osteogenic supplements, 
      showing similar results for all the peptide variants analyzed in this study.
FAU - Ramis, Joana Maria
AU  - Ramis JM
AD  - Group of Cell Therapy and Tissue Engineering, Research Institute on Health 
      Sciences-IUNICS, University of the Balearic Islands, Palma de Mallorca, Spain.
FAU - Rubert, Marina
AU  - Rubert M
FAU - Vondrasek, Jiri
AU  - Vondrasek J
FAU - Gaya, Antoni
AU  - Gaya A
FAU - Lyngstadaas, Staale Petter
AU  - Lyngstadaas SP
FAU - Monjo, Marta
AU  - Monjo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120319
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Biomarkers)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Culture Media)
RN  - 0 (Dental Enamel Proteins)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (enamel matrix proteins)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Biomarkers/metabolism
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Lineage/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Culture Media
MH  - Dental Enamel Proteins/*chemistry
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mesenchymal Stem Cells/*cytology/drug effects/enzymology
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Osteogenesis/*drug effects/genetics
MH  - Peptides/chemistry/*pharmacology
MH  - Proline/*chemistry
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2012/03/21 06:00
MHDA- 2012/09/27 06:00
CRDT- 2012/03/21 06:00
PHST- 2012/03/21 06:00 [entrez]
PHST- 2012/03/21 06:00 [pubmed]
PHST- 2012/09/27 06:00 [medline]
AID - 10.1089/ten.tea.2011.0404 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2012 Jun;18(11-12):1253-63. doi: 10.1089/ten.tea.2011.0404. 
      Epub 2012 Mar 19.