PMID- 22429262
OWN - NLM
STAT- MEDLINE
DCOM- 20120926
LR  - 20211021
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 18
IP  - 11-12
DP  - 2012 Jun
TI  - A comparison of the functionality and in vivo phenotypic stability of 
      cartilaginous tissues engineered from different stem cell sources.
PG  - 1161-70
LID - 10.1089/ten.TEA.2011.0544 [doi]
AB  - Joint-derived stem cells are a promising alternative cell source for cartilage 
      repair therapies that may overcome many of the problems associated with the use 
      of primary chondrocytes (CCs). The objective of this study was to compare the in 
      vitro functionality and in vivo phenotypic stability of cartilaginous tissues 
      engineered using bone marrow-derived stem cells (BMSCs) and joint tissue-derived 
      stem cells following encapsulation in agarose hydrogels. Culture-expanded BMSCs, 
      fat pad-derived stem cells (FPSCs), and synovial membrane-derived stem cells 
      (SDSCs) were encapsulated in agarose and maintained in a chondrogenic medium 
      supplemented with transforming growth factor-beta3. After 21 days of culture, 
      constructs were either implanted subcutaneously into the back of nude mice for an 
      additional 28 days or maintained for a similar period in vitro in either 
      chondrogenic or hypertrophic media formulations. After 49 days of in vitro 
      culture in chondrogenic media, SDSC constructs accumulated the highest levels of 
      sulfated glycosaminoglycan (sGAG) ( approximately 2.8% w/w) and collagen ( approximately 1.8% w/w) and were 
      mechanically stiffer than constructs engineered using other cell types. After 
      subcutaneous implantation in nude mice, sGAG content significantly decreased for 
      all stem cell-seeded constructs, while no significant change was observed in the 
      control constructs engineered using primary CCs, indicating that the in vitro 
      chondrocyte-like phenotype generated in all stem cell-seeded agarose constructs 
      was transient. FPSCs and SDSCs appeared to undergo fibrous dedifferentiation or 
      resorption, as evident from increased collagen type I staining and a dramatic 
      loss in sGAG content. BMSCs followed a more endochondral pathway with increased 
      type X collagen expression and mineralization of the engineered tissue. In 
      conclusion, while joint tissue-derived stem cells possess a strong intrinsic 
      chondrogenic capacity, further studies are needed to identify the factors that 
      will lead to the generation of a more stable chondrogenic phenotype.
FAU - Vinardell, Tatiana
AU  - Vinardell T
AD  - Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Dublin 
      2, Ireland.
FAU - Sheehy, Eamon J
AU  - Sheehy EJ
FAU - Buckley, Conor T
AU  - Buckley CT
FAU - Kelly, Daniel J
AU  - Kelly DJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120427
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Culture Media)
RN  - 0 (Glycosaminoglycans)
RN  - 268AW7000T (A73025)
RN  - 9007-34-5 (Collagen)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cartilage/drug effects/*physiology
MH  - Chondrocytes/cytology/drug effects/metabolism
MH  - Chondrogenesis/drug effects
MH  - Collagen/metabolism
MH  - Culture Media/pharmacology
MH  - Elastic Modulus/drug effects
MH  - Glycosaminoglycans/metabolism
MH  - Hypertrophy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Phenotype
MH  - Sepharose/pharmacology
MH  - Staining and Labeling
MH  - Stem Cells/*cytology
MH  - Sus scrofa
MH  - Synovial Membrane/cytology
MH  - Tissue Engineering/*methods
PMC - PMC3360504
EDAT- 2012/03/21 06:00
MHDA- 2012/09/27 06:00
PMCR- 2013/06/01
CRDT- 2012/03/21 06:00
PHST- 2012/03/21 06:00 [entrez]
PHST- 2012/03/21 06:00 [pubmed]
PHST- 2012/09/27 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2011.0544 [pii]
AID - 10.1089/ten.TEA.2011.0544 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2012 Jun;18(11-12):1161-70. doi: 10.1089/ten.TEA.2011.0544. 
      Epub 2012 Apr 27.