PMID- 22431042
OWN - NLM
STAT- MEDLINE
DCOM- 20150709
LR  - 20181201
IS  - 1006-7248 (Print)
IS  - 1006-7248 (Linking)
VI  - 21
IP  - 1
DP  - 2012 Feb
TI  - [The study of bone formation of hUCMSCs compounded with beta-TCP biological ceramic 
      in vitro and in vivo].
PG  - 24-30
AB  - PURPOSE: To observe the osteogenesis of the construct combining human umbilical 
      cord mesenchymal stem cells (hUCMSCs) with scaffold beta-TCP biological ceramic both 
      in vitro and in vivo. METHODS: A composite was constructed combining hUCMSCs with 
      beta-TCP.The cellular capability of adhesion and growth on scaffold were 
      investigated visually by scanning electron microscopy (SEM), specific 
      immunofluorescence staining, MTT and ALP. The constructs were implanted in nude 
      mice for osteogenic potential. The animals were divided into 3 groups: Group 1 
      (pure beta-TCP), Group 2 (the construct combining hUCMSCs with scaffold beta-TCP was 
      induced to bone in vitro for 2 weeks), Group 3 (simple implantation of the 
      hUCMSCs which was induced to bone in vitro for 2 weeks). 2 months after 
      implantation, the specimens were harvested for gross observation, radiographic 
      examination and histological observation. SPSS 16.0 software package was used for 
      statistical analysis through repeated measures analysis of variances. RESULTS: 
      Cells can be seen attached in the scaffold after 4 hours of the implantation of 
      HUCMSCs, a large number of cells reproduce in the scaffold after 1 week. After 2 
      months of implantation in the nude mice, the group of hUCMSCs and beta-TCP 
      incubation had the highest radiodensity. 2 months after implantation, the group 
      of hUCMSCs and beta-TCP had irregular new bone and vascular formation, beta-TCP group 
      had no obvious new bone and blood vessel formation. 2 months after implantation, 
      hUCMSCs and beta-TCP group had a lot of collagen formation, while beta-TCP group had no 
      obvious formation of collagen by using masson staining. 2 months after 
      implantation, hUCMSCs and beta-TCP group had a large number of osteoid formation, 
      while beta-TCP group had no obvious formation of osteoid by using VG staining. 
      CONCLUSIONS: hUCMSCs and beta-TCP have good biocompatibility, the formation of new 
      bone and blood vessels can be seen after 2 months of implantation of the 
      construct into mice.
FAU - Guo, Yu-cheng
AU  - Guo YC
AD  - Department of Orthodontics, Stomatological Hospital of Xi'an Jiaotong University, 
      Shanxi Province, China. xjtu-guoyucheng@163.com
FAU - Wang, Fei
AU  - Wang F
FAU - Zhou, Hong
AU  - Zhou H
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Shanghai Kou Qiang Yi Xue
JT  - Shanghai kou qiang yi xue = Shanghai journal of stomatology
JID - 101090220
RN  - 0 (Calcium Phosphates)
RN  - 0 (beta-tricalcium phosphate)
SB  - IM
MH  - Animals
MH  - Calcium Phosphates
MH  - *Ceramics
MH  - Humans
MH  - In Vitro Techniques
MH  - Mesenchymal Stem Cells
MH  - Mice
MH  - Mice, Nude
MH  - *Osteogenesis
MH  - Tissue Engineering
EDAT- 2012/03/21 06:00
MHDA- 2015/07/15 06:00
CRDT- 2012/03/21 06:00
PHST- 2012/03/21 06:00 [entrez]
PHST- 2012/03/21 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
PST - ppublish
SO  - Shanghai Kou Qiang Yi Xue. 2012 Feb;21(1):24-30.