PMID- 22431394
OWN - NLM
STAT- MEDLINE
DCOM- 20121005
LR  - 20211203
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 90
IP  - 8
DP  - 2012 Aug
TI  - Effects of pyridoxine on a high-fat diet-induced reduction of cell proliferation 
      and neuroblast differentiation depend on cyclic adenosine monophosphate response 
      element binding protein in the mouse dentate gyrus.
PG  - 1615-25
LID - 10.1002/jnr.23035 [doi]
AB  - In this study, we challenged pyridoxine to mice fed a high-fat diet (HFD) and 
      investigated the effects of pyridoxine on HFD-induced phenotypes such as blood 
      glucose, reduction of cell proliferation and neuroblast differentiation in the 
      dentate gyrus using Ki67 and doublecortin (DCX), respectively. Mice were fed a 
      commercially available low-fat diet (LFD) as control diet or HFD (60% fat) for 8 
      weeks. After 5 weeks of LFD or HFD treatment, 350 mg/kg pyridoxine was 
      administered for 3 weeks. The administration of pyridoxine significantly 
      decreased body weight in the HFD-treated group. In addition, there were no 
      significant differences in hepatic histology and pancreatic 
      insulin-immunoreactive (-ir) and glucagon-ir cells of the HFD-treated group after 
      pyridoxine treatment. In the HFD-fed group, Ki67-positive nuclei and DCX-ir 
      neuroblasts were significantly decreased in the dentate gyrus compared with those 
      in the LFD-fed mice. However, the administration of pyridoxine significantly 
      increased Ki67-positive nuclei and DCX-ir neuroblasts in the dentate gyrus in 
      both LFD- and HFD-fed mice. In addition, the administration of pyridoxine 
      significantly increased the protein levels of glutamic acid decarboxylase 67 
      (GAD67) and brain-derived neurotrophic factor (BDNF) and the immunoreactivity of 
      phosphorylated cyclic AMP response element binding protein (pCREB) compared with 
      the vehicle-treated LFD- and HFD-fed mice. In contrast, the administration of 
      pyridoxine significantly decreased HFD-induced malondialdehyde (MDA) levels in 
      the hippocampus. These results showed that pyridoxine supplement reduced the 
      HFD-induced reduction of cell proliferation and neuroblast differentiation in the 
      dentate gyrus via controlling the levels of GAD67, pCREB, BDNF, and MDA.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Yoo, Dae Young
AU  - Yoo DY
AD  - Department of Anatomy and Cell Biology, College of Veterinary Medicine, and 
      Research Institute for Veterinary Science, Seoul National University, Seoul, 
      South Korea.
FAU - Kim, Woosuk
AU  - Kim W
FAU - Yoo, Ki-Yeon
AU  - Yoo KY
FAU - Nam, Sung Min
AU  - Nam SM
FAU - Chung, Jin Young
AU  - Chung JY
FAU - Yoon, Yeo Sung
AU  - Yoon YS
FAU - Won, Moo-Ho
AU  - Won MH
FAU - Hwang, In Koo
AU  - Hwang IK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120319
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Dcx protein, mouse)
RN  - 0 (Doublecortin Protein)
RN  - 12001-76-2 (Vitamin B Complex)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - KV2JZ1BI6Z (Pyridoxine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Body Weight/drug effects
MH  - CREB-Binding Protein/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Dentate Gyrus/*drug effects/metabolism
MH  - Diet, High-Fat/*adverse effects
MH  - Doublecortin Protein
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neural Stem Cells/*drug effects/metabolism
MH  - Pyridoxine/*pharmacology
MH  - Vitamin B Complex/*pharmacology
EDAT- 2012/03/21 06:00
MHDA- 2012/10/06 06:00
CRDT- 2012/03/21 06:00
PHST- 2011/06/24 00:00 [received]
PHST- 2011/12/28 00:00 [revised]
PHST- 2012/01/07 00:00 [accepted]
PHST- 2012/03/21 06:00 [entrez]
PHST- 2012/03/21 06:00 [pubmed]
PHST- 2012/10/06 06:00 [medline]
AID - 10.1002/jnr.23035 [doi]
PST - ppublish
SO  - J Neurosci Res. 2012 Aug;90(8):1615-25. doi: 10.1002/jnr.23035. Epub 2012 Mar 19.