PMID- 22431997
OWN - NLM
STAT- MEDLINE
DCOM- 20120713
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 3
DP  - 2012
TI  - Dynamics of adrenal steroids are related to variations in Th1 and Treg 
      populations during Mycobacterium tuberculosis infection in HIV positive persons.
PG  - e33061
LID - 10.1371/journal.pone.0033061 [doi]
LID - e33061
AB  - Tuberculosis (TB) remains the most frequent cause of illness and death from an 
      infectious agent, and its interaction with HIV has devastating effects. We 
      determined plasma levels of dehydroepiandrosterone (DHEA), its circulating form 
      DHEA-suphate (DHEA-s) and cortisol in different stages of M. tuberculosis 
      infection, and explored their role on the Th1 and Treg populations during 
      different scenarios of HIV-TB coinfection, including the immune reconstitution 
      inflammatory syndrome (IRIS), a condition related to antiretroviral treatment. 
      DHEA levels were diminished in HIV-TB and HIV-TB IRIS patients compared to 
      healthy donors (HD), HIV+ individuals and HIV+ individuals with latent TB 
      (HIV-LTB), whereas dehydroepiandrosterone sulfate (DHEA-s) levels were markedly 
      diminished in HIV-TB IRIS individuals. HIV-TB and IRIS patients presented a 
      cortisol/DHEA ratio significantly higher than HIV+, HIV-LTB and HD individuals. A 
      positive correlation was observed between DHEA-s and CD4 count among HIV-TB 
      individuals. Conversely, cortisol plasma level inversely correlated with CD4 
      count within HIV-TB individuals. M. tuberculosis-specific Th1 lymphocyte count 
      was increased after culturing PBMC from HIV-TB individuals in presence of DHEA. 
      We observed an inverse correlation between DHEA-s plasma level and Treg frequency 
      in co-infected individuals, and CD4+FoxP3+ Treg frequency was increased in HIV-TB 
      and IRIS patients compared to other groups. Strikingly, we observed a prominent 
      CD4+CD25-FoxP3+ population across HIV-TB and HIV-TB IRIS patients, which 
      frequency correlated with DHEA plasma level. Finally, DHEA treatment negatively 
      regulated FoxP3 expression without altering Treg frequency in co-infected 
      patients. These data suggest an enhancing role for DHEA in the immune response 
      against M. tuberculosis during HIV-TB coinfection and IRIS.
FAU - Quiroga, Maria Florencia
AU  - Quiroga MF
AD  - Department of Microbiology, National Reference Center for AIDS, University of 
      Buenos Aires School of Medicine, Buenos Aires, Argentina. fquiroga@fmed.uba.ar
FAU - Angerami, Matias Tomas
AU  - Angerami MT
FAU - Santucci, Natalia
AU  - Santucci N
FAU - Ameri, Diego
AU  - Ameri D
FAU - Francos, Jose Luis
AU  - Francos JL
FAU - Wallach, Jorge
AU  - Wallach J
FAU - Sued, Omar
AU  - Sued O
FAU - Cahn, Pedro
AU  - Cahn P
FAU - Salomon, Horacio
AU  - Salomon H
FAU - Bottasso, Oscar
AU  - Bottasso O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120314
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Steroids)
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adrenal Glands/drug effects/*metabolism
MH  - Adult
MH  - Coinfection/blood/complications/immunology
MH  - Dehydroepiandrosterone/pharmacology
MH  - Female
MH  - Forkhead Transcription Factors/metabolism
MH  - HIV Infections/blood/complications/*immunology/microbiology
MH  - Humans
MH  - Immune Reconstitution Inflammatory Syndrome/blood/complications/immunology
MH  - Interferon-gamma/immunology
MH  - Interleukin-2 Receptor alpha Subunit/metabolism
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/drug effects/*physiology
MH  - Steroids/blood/*metabolism
MH  - T-Lymphocytes, Regulatory/drug effects/*microbiology
MH  - Th1 Cells/drug effects/*immunology
MH  - Tuberculosis/blood/complications/*immunology
PMC - PMC3303789
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/03/21 06:00
MHDA- 2012/07/14 06:00
PMCR- 2012/03/14
CRDT- 2012/03/21 06:00
PHST- 2011/11/17 00:00 [received]
PHST- 2012/02/03 00:00 [accepted]
PHST- 2012/03/21 06:00 [entrez]
PHST- 2012/03/21 06:00 [pubmed]
PHST- 2012/07/14 06:00 [medline]
PHST- 2012/03/14 00:00 [pmc-release]
AID - PONE-D-11-23005 [pii]
AID - 10.1371/journal.pone.0033061 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(3):e33061. doi: 10.1371/journal.pone.0033061. Epub 2012 Mar 14.