PMID- 22434605
OWN - NLM
STAT- MEDLINE
DCOM- 20121016
LR  - 20151119
IS  - 1552-4957 (Electronic)
IS  - 1552-4949 (Linking)
VI  - 82
IP  - 4
DP  - 2012 Jul
TI  - Expression of coagulation factor XIII subunit A in acute promyelocytic leukemia.
PG  - 209-16
LID - 10.1002/cyto.b.21019 [doi]
AB  - Leukemic cells often express markers, which are not characteristic of their 
      particular cell lineage. In this study, we identified the "A" subunit of 
      coagulation factor XIII (FXIII-A) in leukemic promyelocytes in de novo AML M3 
      cases. The cytoplasmic presence of factor XIII-A has previously been shown only 
      in platelets/megakaryocytes and monocytes/macrophages. Furthermore, more recently 
      we described the presence of FXIII-A in leukemic lymphoblasts. We studied 14 
      patients with this rare type of acute leukemia in a period of 4 years and 
      investigated their bone marrow samples by 3-color flow cytometry upon diagnosis, 
      mainly focusing on FXIII-A expression of leukemic cells. We detected FXIII-A also 
      by ELISA, Western-blot, and confocal laser scanning microscopy. This was a 
      homogenous group of AML M3 patients with translocation t(15;17)(q22;q21) detected 
      by fluorescence in situ hybridization (FISH). In 10 out of 14 samples, FXIII-A 
      was detectable by flow cytometry and was coexpressed with markers characteristic 
      for leukemic promyleocytes (CD45dim/CD13+/CD33+/CD117+/cyMPO+ and 
      HLA-DR-/CD34-/CD14-/CD15-). Staining for the markers GPIIb and GPIX were 
      negative, and FXIII-A was identified in the cytoplasm of the cells by confocal 
      microscopy in a relatively high quantity, as measured by ELISA. By Western blot 
      analysis we could identify FXIII-A in the native 82 kDa form and in cleaved forms 
      corresponding to cleavage products observed when purified FXIII-A was treated by 
      human neutrophil elastase. This novel expression site of FXIII-A in AML M3 can be 
      considered as a leukemia associated immunophenotype and may have 
      pathophysiological significance.
CI  - Copyright (c) 2012 International Clinical Cytometry Society.
FAU - Simon, Agnes
AU  - Simon A
AD  - Department of Laboratory Medicine, Medical and Health Science Center, University 
      of Debrecen, Debrecen, Hungary.
FAU - Bagoly, Zsuzsa
AU  - Bagoly Z
FAU - Hevessy, Zsuzsanna
AU  - Hevessy Z
FAU - Csathy, Laszlo
AU  - Csathy L
FAU - Katona, Eva
AU  - Katona E
FAU - Vereb, Gyorgy
AU  - Vereb G
FAU - Ujfalusi, Aniko
AU  - Ujfalusi A
FAU - Szerafin, Laszlo
AU  - Szerafin L
FAU - Muszbek, Laszlo
AU  - Muszbek L
FAU - Kappelmayer, Janos
AU  - Kappelmayer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120426
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
RN  - 0 (Biomarkers)
RN  - 0 (Protein Subunits)
RN  - EC 2.3.2.13 (Factor XIIIa)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Blotting, Western
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Factor XIIIa/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Granulocyte Precursor Cells/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Leukemia, Promyelocytic, Acute/*metabolism/pathology
MH  - Male
MH  - Microscopy, Confocal
MH  - Middle Aged
MH  - Protein Subunits/*metabolism
EDAT- 2012/03/22 06:00
MHDA- 2012/10/17 06:00
CRDT- 2012/03/22 06:00
PHST- 2011/09/09 00:00 [received]
PHST- 2012/03/05 00:00 [revised]
PHST- 2012/03/08 00:00 [accepted]
PHST- 2012/03/22 06:00 [entrez]
PHST- 2012/03/22 06:00 [pubmed]
PHST- 2012/10/17 06:00 [medline]
AID - 10.1002/cyto.b.21019 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2012 Jul;82(4):209-16. doi: 10.1002/cyto.b.21019. Epub 
      2012 Apr 26.