PMID- 22435653
OWN - NLM
STAT- MEDLINE
DCOM- 20121226
LR  - 20240418
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 18
IP  - 15-16
DP  - 2012 Aug
TI  - Biofunctionalized calcium phosphate cement to enhance the attachment and 
      osteodifferentiation of stem cells released from fast-degradable alginate-fibrin 
      microbeads.
PG  - 1583-95
LID - 10.1089/ten.TEA.2011.0604 [doi]
AB  - Stem cell-encapsulating microbeads could be mixed into a paste such as calcium 
      phosphate cement (CPC), where the microbeads could protect the cells from the 
      mixing and injection forces. After being placed, the microbeads could quickly 
      degrade to release the cells throughout the scaffold, while creating macropores. 
      The objectives of this study were to (1) construct alginate-fibrin microbeads 
      encapsulating human umbilical cord mesenchymal stem cells (hUCMSCs) embedded in 
      the surface of novel biofunctionalized CPC and (2) investigate microbead 
      degradation, cell release, and osteodifferentiation on CPC. Hydrogel microbeads 
      were fabricated that encapsulated hUCMSCs at 1x10(6) cells/mL. CPC was 
      biofunctionalized with fibronectin (Fn) and Arg-Gly-Asp (RGD). Four scaffolds 
      were tested: CPC control, CPC mixed with Fn, CPC mixed with RGD, and CPC grafted 
      with RGD. The degradable microbeads released hUCMSCs at 7 days, which attached to 
      CPC. Adding Fn or RGD to CPC greatly improved cell attachment. CPC grafted with 
      RGD showed the fastest cell proliferation, with cell density being ninefold that 
      on CPC control. The released hUCMSCs underwent osteodifferentiation. Alkaline 
      phosphatase, osteocalcin, collagen 1, and runt-related transcription factor 2 
      (Runx2) gene expression increased by 10 to 30 fold at 7-21 days, compared with 
      day 1. The released cells on CPC synthesized bone minerals, with the 
      mineralization amount at 21 days being two orders of magnitude higher than that 
      at 7 days. In conclusion, alginate-fibrin microbeads embedded in CPC surface were 
      able to quickly release the hUCMSCs that attached to biofunctionalized CPC. 
      Incorporating Fn and RGD into CPC greatly improved cell function, and CPC grafted 
      with RGD had the fastest cell proliferation. The released cells on CPC 
      differentiated into the osteogenic lineage and synthesized bone minerals. The new 
      biofunctionalized CPC with hUCMSC-encapsulating microbeads is promising for bone 
      regeneration applications.
FAU - Zhou, Hongzhi
AU  - Zhou H
AD  - Biomaterials & Tissue Engineering Division, Department of Endodontics, 
      Prosthodontics and Operative Dentistry, University of Maryland School of 
      Dentistry, Baltimore, MD 21201, USA. hz_zhou@yahoo.com
FAU - Chen, Wenchuan
AU  - Chen W
FAU - Weir, Michael D
AU  - Weir MD
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01DE17974/DE/NIDCR NIH HHS/United States
GR  - R01DE14190/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120514
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Alginates)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Oligopeptides)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9001-31-4 (Fibrin)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Alginates/*pharmacology
MH  - Animals
MH  - Biocompatible Materials/*pharmacology
MH  - Bone Cements/chemistry/*pharmacology
MH  - Calcification, Physiologic/drug effects
MH  - Calcium Phosphates/chemistry/*pharmacology
MH  - Cattle
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Immobilized
MH  - Fibrin/*pharmacology
MH  - Glucuronic Acid/pharmacology
MH  - Hexuronic Acids/pharmacology
MH  - Humans
MH  - Materials Testing
MH  - Mechanical Phenomena/drug effects
MH  - Mesenchymal Stem Cells/cytology/drug effects/metabolism
MH  - Microspheres
MH  - Oligopeptides/pharmacology
MH  - Osteogenesis/*drug effects
MH  - Staining and Labeling
MH  - Stem Cells/*cytology
MH  - Umbilical Cord/cytology
PMC - PMC3419861
EDAT- 2012/03/23 06:00
MHDA- 2012/12/27 06:00
PMCR- 2013/08/01
CRDT- 2012/03/23 06:00
PHST- 2012/03/23 06:00 [entrez]
PHST- 2012/03/23 06:00 [pubmed]
PHST- 2012/12/27 06:00 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2011.0604 [pii]
AID - 10.1089/ten.TEA.2011.0604 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2012 Aug;18(15-16):1583-95. doi: 10.1089/ten.TEA.2011.0604. 
      Epub 2012 May 14.