PMID- 22438294
OWN - NLM
STAT- MEDLINE
DCOM- 20140409
LR  - 20130621
IS  - 1477-0393 (Electronic)
IS  - 0748-2337 (Linking)
VI  - 29
IP  - 6
DP  - 2013 Jul
TI  - HSP70 immune reactivity and TUNEL positivity in the liver of toluene-inhaled and 
      melatonin-treated rats.
PG  - 514-22
LID - 10.1177/0748233712440138 [doi]
AB  - Toluene is a clear, colorless and volatile hydrocarbon that is metabolized in 
      liver, produced free oxygen radicals and can mediate cellular damage. Melatonin 
      which is a pineal gland hormone is a very potent antioxidant. It can make the 
      cellular membrane more durable against oxidative attacks and protect nuclear DNA 
      from oxidative damage. This study aimed to investigate heat shock protein (HSP)70 
      immune reactivity and terminal deoxynucleotidyl transferase-mediated dUTP nick 
      end labeling (TUNEL) positivity (apoptotic activity) in the liver of 
      toluene-inhaled and melatonin-treated rats. A total of 21 adult male Wistar 
      albino rats were divided at random into 3 equal groups. Animals in group I were 
      designated as control. The rats in group II were exposed to toluene 
      (3000 ppm/1 h/day) for 30 days, while the rats in group III were treated with 
      melatonin (10 mg/kg/day, intraperitoneally) plus toluene inhalation. At the end 
      of the 30-day experimental period, all rats were killed by decapitation. Then the 
      liver tissues of rats were removed and tissue specimens were embedded in paraffin 
      blocks. The specimens were stained with periodic acid-schiff (PAS) following 
      routine histological procedures. Sections obtained from paraffin blocks were used 
      for immune detection of TUNEL and HSP70. In light microscopic observations of 
      tissues from toluene-inhaled rats, massive hepatocyte degeneration, ballooning 
      degeneration and decreased PAS positivity were observed. Increased TUNEL 
      positivity and HSP70 immune reactivity were determined in toluene-inhaled group 
      and melatonin treatment decreased all these adverse effects.
FAU - Tas, Ufuk
AU  - Tas U
AD  - Department of Anatomy, Gaziosmanpasa University, Tokat, Turkey. 
      dr_ufuktas@hotmail.com
FAU - Ogeturk, Murat
AU  - Ogeturk M
FAU - Kuloglu, Tuncay
AU  - Kuloglu T
FAU - Sapmaz, Hilal Irmak
AU  - Sapmaz HI
FAU - Kocaman, Nevin
AU  - Kocaman N
FAU - Zararsiz, Ismail
AU  - Zararsiz I
FAU - Sarsilmaz, Mustafa
AU  - Sarsilmaz M
LA  - eng
PT  - Journal Article
DEP - 20120321
PL  - England
TA  - Toxicol Ind Health
JT  - Toxicology and industrial health
JID - 8602702
RN  - 0 (Antioxidants)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 3FPU23BG52 (Toluene)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/drug effects/genetics
MH  - HSP70 Heat-Shock Proteins/*immunology
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Inhalation Exposure
MH  - Liver/*drug effects/immunology/pathology
MH  - Male
MH  - Melatonin/*pharmacology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Statistics, Nonparametric
MH  - Toluene/administration & dosage/*toxicity
OTO - NOTNLM
OT  - HSP70
OT  - TUNEL
OT  - Toluene
OT  - liver
OT  - melatonin
EDAT- 2012/03/23 06:00
MHDA- 2014/04/10 06:00
CRDT- 2012/03/23 06:00
PHST- 2012/03/23 06:00 [entrez]
PHST- 2012/03/23 06:00 [pubmed]
PHST- 2014/04/10 06:00 [medline]
AID - 0748233712440138 [pii]
AID - 10.1177/0748233712440138 [doi]
PST - ppublish
SO  - Toxicol Ind Health. 2013 Jul;29(6):514-22. doi: 10.1177/0748233712440138. Epub 
      2012 Mar 21.