PMID- 22438548
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20240504
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 10
DP  - 2012 May
TI  - The double-stranded RNA bluetongue virus induces type I interferon in 
      plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling 
      pathway.
PG  - 5817-28
LID - 10.1128/JVI.06716-11 [doi]
AB  - Dendritic cells (DCs), especially plasmacytoid DCs (pDCs), produce large amounts 
      of alpha/beta interferon (IFN-alpha/beta) upon infection with DNA or RNA viruses, which 
      has impacts on the physiopathology of the viral infections and on the quality of 
      the adaptive immunity. However, little is known about the IFN-alpha/beta production by 
      DCs during infections by double-stranded RNA (dsRNA) viruses. We present here 
      novel information about the production of IFN-alpha/beta induced by bluetongue virus 
      (BTV), a vector-borne dsRNA Orbivirus of ruminants, in sheep primary DCs. We 
      found that BTV induced IFN-alpha/beta in skin lymph and in blood in vivo. Although BTV 
      replicated in a substantial fraction of the conventional DCs (cDCs) and pDCs in 
      vitro, only pDCs responded to BTV by producing a significant amount of IFN-alpha/beta. 
      BTV replication in pDCs was not mandatory for IFN-alpha/beta production since it was 
      still induced by UV-inactivated BTV (UV-BTV). Other inflammatory cytokines, 
      including tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and 
      IL-12p40, were also induced by UV-BTV in primary pDCs. The induction of IFN-alpha/beta 
      required endo-/lysosomal acidification and maturation. However, despite being an 
      RNA virus, UV-BTV did not signal through Toll-like receptor 7 (TLR7) for IFN-alpha/beta 
      induction. In contrast, pathways involving the MyD88 adaptor and kinases 
      dsRNA-activated protein kinase (PKR) and stress-activated protein kinase 
      (SAPK)/Jun N-terminal protein kinase (JNK) were implicated. This work highlights 
      the importance of pDCs for the production of innate immunity cytokines induced by 
      a dsRNA virus, and it shows that a dsRNA virus can induce IFN-alpha/beta in pDCs via a 
      novel TLR-independent and Myd88-dependent pathway. These findings have 
      implications for the design of efficient vaccines against dsRNA viruses.
FAU - Ruscanu, Suzana
AU  - Ruscanu S
AD  - Virologie et Immunologie Moleculaires, UR892 INRA, Jouy-en-Josas, France.
FAU - Pascale, Florentina
AU  - Pascale F
FAU - Bourge, Mickael
AU  - Bourge M
FAU - Hemati, Behzad
AU  - Hemati B
FAU - Elhmouzi-Younes, Jamila
AU  - Elhmouzi-Younes J
FAU - Urien, Celine
AU  - Urien C
FAU - Bonneau, Michel
AU  - Bonneau M
FAU - Takamatsu, Haru
AU  - Takamatsu H
FAU - Hope, Jayne
AU  - Hope J
FAU - Mertens, Peter
AU  - Mertens P
FAU - Meyer, Gilles
AU  - Meyer G
FAU - Stewart, Meredith
AU  - Stewart M
FAU - Roy, Polly
AU  - Roy P
FAU - Meurs, Eliane F
AU  - Meurs EF
FAU - Dabo, Stephanie
AU  - Dabo S
FAU - Zientara, Stephan
AU  - Zientara S
FAU - Breard, Emmanuel
AU  - Breard E
FAU - Sailleau, Corinne
AU  - Sailleau C
FAU - Chauveau, Emilie
AU  - Chauveau E
FAU - Vitour, Damien
AU  - Vitour D
FAU - Charley, Bernard
AU  - Charley B
FAU - Schwartz-Cornil, Isabelle
AU  - Schwartz-Cornil I
LA  - eng
GR  - BB/F00852X/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BBS/E/I/00001441/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00001709/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120321
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Cytokines)
RN  - 0 (Interferon Type I)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Interleukin-1)
RN  - 0 (TIRAP protein, human)
RN  - 0 (Toll-Like Receptor 7)
RN  - 0 (Toll-Like Receptor 8)
SB  - IM
MH  - Animals
MH  - Bluetongue/genetics/*immunology/virology
MH  - Bluetongue virus/genetics/*immunology/physiology
MH  - Cells, Cultured
MH  - Cytokines/genetics/immunology
MH  - Dendritic Cells/*immunology/virology
MH  - Female
MH  - Immunity, Innate
MH  - Interferon Type I/genetics/*immunology
MH  - Membrane Glycoproteins
MH  - Myeloid Differentiation Factor 88/genetics/*immunology
MH  - Receptors, Interleukin-1
MH  - Sheep/immunology/virology
MH  - Signal Transduction
MH  - Toll-Like Receptor 7/genetics/*immunology
MH  - Toll-Like Receptor 8/genetics/*immunology
PMC - PMC3347300
EDAT- 2012/03/23 06:00
MHDA- 2012/06/19 06:00
PMCR- 2012/11/01
CRDT- 2012/03/23 06:00
PHST- 2012/03/23 06:00 [entrez]
PHST- 2012/03/23 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - JVI.06716-11 [pii]
AID - 06716-11 [pii]
AID - 10.1128/JVI.06716-11 [doi]
PST - ppublish
SO  - J Virol. 2012 May;86(10):5817-28. doi: 10.1128/JVI.06716-11. Epub 2012 Mar 21.