PMID- 22438991
OWN - NLM
STAT- MEDLINE
DCOM- 20120813
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 3
DP  - 2012
TI  - Developmental regulation and spatiotemporal redistribution of the sumoylation 
      machinery in the rat central nervous system.
PG  - e33757
LID - 10.1371/journal.pone.0033757 [doi]
LID - e33757
AB  - BACKGROUND: Small Ubiquitin-like MOdifier protein (SUMO) is a key regulator of 
      nuclear functions but little is known regarding the role of the 
      post-translational modification sumoylation outside of the nucleus, particularly 
      in the Central Nervous System (CNS). METHODOLOGY/PRINCIPAL FINDINGS: Here, we 
      report that the expression levels of SUMO-modified substrates as well as the 
      components of the sumoylation machinery are temporally and spatially regulated in 
      the developing rat brain. Interestingly, while the overall sumoylation is 
      decreasing during brain development, there are progressively more SUMO substrates 
      localized at synapses. This increase is correlated with a differential 
      redistribution of the sumoylation machinery into dendritic spines during neuronal 
      maturation. CONCLUSIONS/SIGNIFICANCE: Overall, our data clearly demonstrate that 
      the sumoylation process is developmentally regulated in the brain with high 
      levels of nuclear sumoylation early in the development suggesting a role for this 
      post-translational modification during the synaptogenesis period and a 
      redistribution of the SUMO system towards dendritic spines at a later 
      developmental stage to modulate synaptic protein function.
FAU - Loriol, Celine
AU  - Loriol C
AD  - Institut de Pharmacologie Moleculaire et Cellulaire, Centre National de la 
      Recherche Scientifique, Valbonne, France.
FAU - Parisot, Josephine
AU  - Parisot J
FAU - Poupon, Gwenola
AU  - Poupon G
FAU - Gwizdek, Carole
AU  - Gwizdek C
FAU - Martin, Stephane
AU  - Martin S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120316
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 6.3.2.- (ubiquitin-conjugating enzyme UBC9)
SB  - IM
MH  - Animals
MH  - Brain/embryology/growth & development/metabolism
MH  - Cells, Cultured
MH  - Central Nervous System/embryology/growth & development/*metabolism
MH  - Dendrites/metabolism
MH  - Hippocampus/cytology/metabolism
MH  - Neurogenesis/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Subcellular Fractions/metabolism
MH  - *Sumoylation
MH  - Synapses/metabolism
MH  - Tissue Distribution
MH  - Ubiquitin-Conjugating Enzymes/chemistry/metabolism
PMC - PMC3306303
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/03/23 06:00
MHDA- 2012/08/14 06:00
PMCR- 2012/03/16
CRDT- 2012/03/23 06:00
PHST- 2012/01/10 00:00 [received]
PHST- 2012/02/21 00:00 [accepted]
PHST- 2012/03/23 06:00 [entrez]
PHST- 2012/03/23 06:00 [pubmed]
PHST- 2012/08/14 06:00 [medline]
PHST- 2012/03/16 00:00 [pmc-release]
AID - PONE-D-12-00827 [pii]
AID - 10.1371/journal.pone.0033757 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(3):e33757. doi: 10.1371/journal.pone.0033757. Epub 2012 Mar 16.