PMID- 22444464
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20220330
IS  - 1751-7311 (Print)
IS  - 1751-7311 (Linking)
VI  - 1
IP  - 5
DP  - 2007 Jun
TI  - The use of a tannin crude extract from Cistus ladanifer L. to protect soya-bean 
      protein from degradation in the rumen.
PG  - 645-50
LID - 10.1017/S1751731107689745 [doi]
AB  - Cistus ladanifer L. (CL) is a perennial shrub abundant in dry woods and dry land 
      of Mediterranean zone, with high level of tannins. Tannins bind to protein, 
      preventing its degradation in the digestive compartments. This tannin/protein 
      complex may be advantageous when partially protecting good-quality feed protein 
      from excessive rumen protein degradation. The objective of this trial was to use 
      a CL phenol crude extract to prevent excessive rumen degradation of soya-bean 
      meal protein. The phenolic compounds were extracted using an acetone/water 
      solution (70:30, v/v). Soya-bean meal was then treated with this crude CL 
      extract, containing 640 g of total phenols (TP) per kg of dry matter (DM), in 
      order to obtain mixtures with 0, 12.5, 25, 50, 100 and 150 g of TP per kg DM. 
      Three rumen-cannulated rams were used to assess in sacco rumen degradability of 
      DM and nitrogen (N). The three-step in vitro procedure was used to determine 
      intestinal digestibility. Increasing extract concentrations quadratically 
      decreased the N-soluble fraction a (R2 = 0.96, P = 0.0001) and increased the 
      non-soluble degradable fraction b (R2 = 0.92, P = 0.005). The rate of degradation 
      c linearly decreased with CL extract doses (R2 = 0.44, P = 0.0065). For the 
      effective rumen degradability of N, a linear reduction (R2 = 0.94, P < 0.0001) 
      was observed. The in vitro intestinal digestibility of protein (ivID) 
      quadratically decreased (R2 = 0.99, P < 0.0001) with TP inclusion and the rumen 
      undegradable protein (RUP) showed a quadratic increase (R2 = 0.94, P = 0.0417). 
      Total intestinal protein availability, computed from the RUP and ivID, linearly 
      decreased with TP inclusion level (R2 = 0.45, P = 0.0033).
FAU - Dentinho, M T P
AU  - Dentinho MT
AD  - Estacao Zootecnica Nacional, Fonte Boa, 2005-048 Vale de Santarem, Portugal.
FAU - Moreira, O C
AU  - Moreira OC
FAU - Pereira, M S
AU  - Pereira MS
FAU - Bessa, R J B
AU  - Bessa RJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Animal
JT  - Animal : an international journal of animal bioscience
JID - 101303270
EDAT- 2007/06/01 00:00
MHDA- 2007/06/01 00:01
CRDT- 2012/03/27 06:00
PHST- 2012/03/27 06:00 [entrez]
PHST- 2007/06/01 00:00 [pubmed]
PHST- 2007/06/01 00:01 [medline]
AID - S1751731107689745 [pii]
AID - 10.1017/S1751731107689745 [doi]
PST - ppublish
SO  - Animal. 2007 Jun;1(5):645-50. doi: 10.1017/S1751731107689745.