PMID- 22445830
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20151119
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 413
IP  - 13-14
DP  - 2012 Jul 11
TI  - Reference interval computation: which method (not) to choose?
PG  - 1107-14
LID - 10.1016/j.cca.2012.03.005 [doi]
AB  - BACKGROUND: When different methods are applied to reference interval (RI) 
      calculation the results can sometimes be substantially different, especially for 
      small reference groups. If there are no reliable RI data available, there is no 
      way to confirm which method generates results closest to the true RI. METHODS: We 
      randomly drawn samples obtained from a public database for 33 markers. For each 
      sample, RIs were calculated by bootstrapping, parametric, and Box-Cox transformed 
      parametric methods. Results were compared to the values of the population RI. 
      RESULTS: For approximately half of the 33 markers, results of all 3 methods were 
      within 3% of the true reference value. For other markers, parametric results were 
      either unavailable or deviated considerably from the true values. The transformed 
      parametric method was more accurate than bootstrapping for sample size of 60, 
      very close to bootstrapping for sample size 120, but in some cases unavailable. 
      CONCLUSIONS: We recommend against using parametric calculations to determine RIs. 
      The transformed parametric method utilizing Box-Cox transformation would be 
      preferable way of RI calculation, if it satisfies normality test. If not, the 
      bootstrapping is always available, and is almost as accurate and precise as the 
      transformed parametric method.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Pavlov, Igor Y
AU  - Pavlov IY
AD  - ARUP Institute for Clinical and Experimental Pathology, Department of Pathology, 
      University of Utah School of Medicine, Salt Lake City, Utah 84108, USA. 
      igor.pavlov@aruplab.com
FAU - Wilson, Andrew R
AU  - Wilson AR
FAU - Delgado, Julio C
AU  - Delgado JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120314
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Biomarkers
MH  - Computer Simulation
MH  - *Data Interpretation, Statistical
MH  - Databases, Factual
MH  - Humans
MH  - Middle Aged
MH  - Random Allocation
MH  - Reference Values
MH  - Young Adult
EDAT- 2012/03/27 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/03/27 06:00
PHST- 2012/02/07 00:00 [received]
PHST- 2012/03/02 00:00 [revised]
PHST- 2012/03/07 00:00 [accepted]
PHST- 2012/03/27 06:00 [entrez]
PHST- 2012/03/27 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - S0009-8981(12)00115-5 [pii]
AID - 10.1016/j.cca.2012.03.005 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2012 Jul 11;413(13-14):1107-14. doi: 10.1016/j.cca.2012.03.005. 
      Epub 2012 Mar 14.