PMID- 22445893
OWN - NLM
STAT- MEDLINE
DCOM- 20120601
LR  - 20180815
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 420
IP  - 3
DP  - 2012 Apr 13
TI  - Thyroglobulin (Tg) activates MAPK pathway to induce thyroid cell growth in the 
      absence of TSH, insulin and serum.
PG  - 611-5
LID - 10.1016/j.bbrc.2012.03.046 [doi]
AB  - The growth of thyroid cells is tightly regulated by thyroid stimulating hormone 
      (TSH) through the cyclic adenosine 3', 5'-monophosphate (cAMP) signaling pathway 
      by potentiating the mitogenic activity of insulin and insulin-like growth factors 
      (IGFs). However, we recently reported that thyroglobulin (Tg), a major product of 
      the thyroid, also induces the growth of thyroid cells cultured in 0.2% serum in 
      the absence of TSH and insulin. In this report, we demonstrate that Tg induced 
      phosphorylation of molecules of the c-Raf/MEK/ERK pathway of the 
      mitogen-activated protein kinase (MAPK). The MEK-1/2 inhibitor PD98059 suppressed 
      Tg-induced phosphorylation of ERK1/2 and reduced bromodeoxyuridine (BrdU) 
      incorporation. Tg also induced expression of the essential transcriptional 
      factors c-Myc, c-Fos and c-Jun and phosphorylation of the retinoblastoma (Rb) 
      protein. The present results, together with the previous report, suggest that Tg 
      utilizes multiple signaling cascades to induce thyroid cell growth independent of 
      TSH/cAMP stimulation.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Sue, Mariko
AU  - Sue M
AD  - Laboratory of Molecular Diagnostics, Department of Mycobacteriology, Leprosy 
      Research Center, National Institute of Infectious Diseases, Higashimurayama, 
      Tokyo 189-0002, Japan.
FAU - Hayashi, Moyuru
AU  - Hayashi M
FAU - Kawashima, Akira
AU  - Kawashima A
FAU - Akama, Takeshi
AU  - Akama T
FAU - Tanigawa, Kazunari
AU  - Tanigawa K
FAU - Yoshihara, Aya
AU  - Yoshihara A
FAU - Hara, Takeshi
AU  - Hara T
FAU - Ishido, Yuko
AU  - Ishido Y
FAU - Ito, Tetsuhide
AU  - Ito T
FAU - Takahashi, Shin-Ichiro
AU  - Takahashi S
FAU - Ishii, Norihisa
AU  - Ishii N
FAU - Suzuki, Koichi
AU  - Suzuki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120316
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Flavonoids)
RN  - 0 (Insulin)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 9002-71-5 (Thyrotropin)
RN  - 9010-34-8 (Thyroglobulin)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-raf)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Proliferation/*drug effects
MH  - Culture Media, Serum-Free/pharmacology
MH  - DNA Replication/drug effects
MH  - Enzyme Activation
MH  - Extracellular Signal-Regulated MAP Kinases/*biosynthesis
MH  - Flavonoids/pharmacology
MH  - Gene Expression/drug effects
MH  - Insulin/pharmacology
MH  - MAP Kinase Kinase Kinases/*biosynthesis
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - Proto-Oncogene Proteins c-raf/*biosynthesis
MH  - Rats
MH  - Thyroglobulin/*pharmacology
MH  - Thyroid Gland/cytology/*drug effects/enzymology
MH  - Thyrotropin/pharmacology
EDAT- 2012/03/27 06:00
MHDA- 2012/06/02 06:00
CRDT- 2012/03/27 06:00
PHST- 2012/03/08 00:00 [received]
PHST- 2012/03/09 00:00 [accepted]
PHST- 2012/03/27 06:00 [entrez]
PHST- 2012/03/27 06:00 [pubmed]
PHST- 2012/06/02 06:00 [medline]
AID - S0006-291X(12)00493-7 [pii]
AID - 10.1016/j.bbrc.2012.03.046 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Apr 13;420(3):611-5. doi: 
      10.1016/j.bbrc.2012.03.046. Epub 2012 Mar 16.