PMID- 22446309
OWN - NLM
STAT- MEDLINE
DCOM- 20130128
LR  - 20211021
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 27
IP  - 3
DP  - 2012
TI  - Diversity of cultivable methane-oxidizing bacteria in microsites of a rice paddy 
      field: investigation by cultivation method and fluorescence in situ hybridization 
      (FISH).
PG  - 278-87
AB  - The diversity of cultivable methane-oxidizing bacteria (MOB) in the rice paddy 
      field ecosystem was investigated by combined culture-dependent and fluorescence 
      in situ hybridization (FISH) techniques. Seven microsites of a Japanese rice 
      paddy field were the focus of the study: floodwater, surface soil, bulk soil, 
      rhizosphere soil, root, basal stem of rice plant, and rice stumps of previous 
      harvest. Based on pmoA gene analysis and transmission electron microscopy (TEM), 
      four type I, and nine type II MOB isolates were obtained from the highest 
      dilution series of enrichment cultures. The type I MOB isolates included a novel 
      species in the genus Methylomonas from floodwater and this is the first type I 
      MOB strain isolated from floodwater of a rice paddy field. In the type I MOB, two 
      isolates from stumps were closely related to Methylomonas spp.; one isolate 
      obtained from rhizosphere soil was most related to 
      Methyloccocus-Methylocaldum-Methylogaea clade. Almost all the type II MOB 
      isolates were related to Methylocystis methanotrophs. FISH confirmed the presence 
      of both types I and II MOB in all the microsites and in the related enrichment 
      cultures. The study reported, for the first time, the diversity of cultivable 
      methanotrophs including a novel species of type I MOB in rice paddy field 
      compartments. Refining growth media and culture conditions, in combination with 
      molecular approaches, will allow us to broaden our knowledge on the MOB community 
      in the rice paddy field ecosystem and consequently to implement strategies for 
      mitigating CH(4) emission from this ecosystem.
FAU - Dianou, Dayeri
AU  - Dianou D
AD  - Centre National de la Recherche Scientifique et Technologique, 03BP7192 
      Ouagadougou, Burkina Faso.
FAU - Ueno, Chihoko
AU  - Ueno C
FAU - Ogiso, Takuya
AU  - Ogiso T
FAU - Kimura, Makoto
AU  - Kimura M
FAU - Asakawa, Susumu
AU  - Asakawa S
LA  - eng
SI  - GENBANK/AB669143
SI  - GENBANK/AB669144
SI  - GENBANK/AB669145
SI  - GENBANK/AB669146
SI  - GENBANK/AB669147
SI  - GENBANK/AB669148
SI  - GENBANK/AB669149
SI  - GENBANK/AB669150
SI  - GENBANK/AB669151
SI  - GENBANK/AB669152
SI  - GENBANK/AB669153
SI  - GENBANK/AB669154
SI  - GENBANK/AB669155
SI  - GENBANK/AB669156
SI  - GENBANK/AB669157
SI  - GENBANK/AB669158
SI  - GENBANK/AB669159
SI  - GENBANK/AB669160
SI  - GENBANK/AB669161
SI  - GENBANK/AB669162
SI  - GENBANK/AB669163
SI  - GENBANK/AB669164
SI  - GENBANK/AB669165
SI  - GENBANK/AB669166
SI  - GENBANK/AB669167
SI  - GENBANK/AB669168
SI  - GENBANK/AB669169
SI  - GENBANK/AB669170
SI  - GENBANK/AB669171
SI  - GENBANK/AB669172
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120323
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Bacteria/*classification/*genetics/isolation & purification/metabolism
MH  - *Biota
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - *Environmental Microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Japan
MH  - Methane/*metabolism
MH  - Microscopy, Electron
MH  - Molecular Sequence Data
MH  - Oryza/*microbiology
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
PMC - PMC4036049
EDAT- 2012/03/27 06:00
MHDA- 2013/01/29 06:00
PMCR- 2012/09/01
CRDT- 2012/03/27 06:00
PHST- 2012/03/27 06:00 [entrez]
PHST- 2012/03/27 06:00 [pubmed]
PHST- 2013/01/29 06:00 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - JST.JSTAGE/jsme2/ME11327 [pii]
AID - 27_278 [pii]
AID - 10.1264/jsme2.me11327 [doi]
PST - ppublish
SO  - Microbes Environ. 2012;27(3):278-87. doi: 10.1264/jsme2.me11327. Epub 2012 Mar 
      23.