PMID- 22447044
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20161125
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 29
IP  - 6
DP  - 2012 Jun
TI  - Protein kinase CK2 mediates peroxynitrite-induced heme oxygenase-1 expression in 
      articular chondrocytes.
PG  - 1039-44
LID - 10.3892/ijmm.2012.949 [doi]
AB  - Heme oxygenase-1 (HO-1) is induced as an adaptive mechanism against oxidative 
      stress in chondrocytes, which play an important role in the maintenance and 
      degradation of cartilage. In the present study, we examined the role of protein 
      kinase casein kinase (CK2) on peroxynitrite-induced expression of HO-1 in primary 
      articular chondrocytes. 3-Muorpholinosydnonimine hydrochloride (SIN-1) has been 
      shown to mediate cell death by activating apoptosis-related molecules in cells. 
      In this study, we used a low concentration of SIN-1 that did not induce apoptosis 
      to elucidate the mechanism by which SIN-1 upregulates HO-1 expression. In 
      chondrocytes, SIN-1 induced HO-1 expression with spontaneous downregulation in a 
      different manner than with high concentrations of SIN-1. Importantly, SIN-1 
      treatment of chondrocytes increased CK2 activation. Additionally, inhibition of 
      CK2 with 4,5,6,7-tetrabromobenzotriazole (TBB) or siRNA did not induce HO-1 
      expression and reduced NF-E2-related factor 2 (Nrf2) accumulation in 
      chondrocytes. Therefore, we examined whether CK2 directly regulates Nrf2, which 
      is a transcription factor that regulates the expression of HO-1. Indeed, TBB 
      treatment inhibited phosphorylation and nuclear translocation of Nrf2 in 
      SIN-1-treated cells. Moreover, using an immunoprecipitation assay, we confirmed 
      that SIN-1 treatment enhanced the interaction between CK2 and Nrf2. Taken 
      together, our findings suggest that peroxynitrite activates Nrf2 via CK2 
      signaling, leading to the upregulation of HO-1 in primary chondrocytes.
FAU - Kim, Kang Mi
AU  - Kim KM
AD  - Department of Microbiology and Immunology, Pusan National University School of 
      Medicine, Yangsan, Gyeongnam 626-870, Republic of Korea.
FAU - Song, Ju Dong
AU  - Song JD
FAU - Chung, Hun Taeg
AU  - Chung HT
FAU - Park, Young Chul
AU  - Park YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120323
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (NF-E2-Related Factor 2)
RN  - 14691-52-2 (Peroxynitrous Acid)
RN  - 5O5U71P6VQ (linsidomine)
RN  - D46583G77X (Molsidomine)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.11.1 (Casein Kinase II)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cartilage, Articular/*cytology
MH  - Casein Kinase II/antagonists & inhibitors/*metabolism
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/drug effects/*enzymology
MH  - Enzyme Induction/drug effects
MH  - Female
MH  - Heme Oxygenase-1/*biosynthesis
MH  - Molsidomine/analogs & derivatives/pharmacology
MH  - NF-E2-Related Factor 2/metabolism
MH  - Peroxynitrous Acid/*pharmacology
MH  - Phosphorylation/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2012/03/27 06:00
MHDA- 2012/08/04 06:00
CRDT- 2012/03/27 06:00
PHST- 2012/01/09 00:00 [received]
PHST- 2012/01/30 00:00 [accepted]
PHST- 2012/03/27 06:00 [entrez]
PHST- 2012/03/27 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
AID - 10.3892/ijmm.2012.949 [doi]
PST - ppublish
SO  - Int J Mol Med. 2012 Jun;29(6):1039-44. doi: 10.3892/ijmm.2012.949. Epub 2012 Mar 
      23.