PMID- 2244784 OWN - NLM STAT- MEDLINE DCOM- 19910103 LR - 20190903 IS - 0302-8933 (Print) IS - 0302-8933 (Linking) VI - 154 IP - 4 DP - 1990 TI - Influence of environmental factors on 2,4-dichlorophenoxyacetic acid degradation by Pseudomonas cepacia isolated from peat. PG - 317-22 AB - A Pseudomonas cepacia, designated strain BRI6001, was isolated from peat by enrichment culture using 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole carbon source. BRI6001 grew at up to 13 mM 2,4-D, and degraded 1 mM 2,4-D at an average starting population density as low as 1.5 cells/ml. Degradation was optimal at acidic pH, but could also be inhibited at low pH, associated with chloride release from the substrate, and the limited buffering capacity of the growth medium. The only metabolite detected during growth on 2,4-D was 2,4-dichlorophenol (2,4-DCP), and degradation of the aromatic nucleus was by intradiol cleavage. Growth lag times prior to the on-set of degradation, and the total time required for degradation, were linearly related to the starting population density and the initial 2,4-D concentration. BRI6001, grown on 2,4-D, oxidized a variety of structurally similar chlorinated aromatic compounds accompanied by stoichiometric chloride release. FAU - Greer, C W AU - Greer CW AD - Environmental Engineering, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec. FAU - Hawari, J AU - Hawari J FAU - Samson, R AU - Samson R LA - eng PT - Journal Article PL - Germany TA - Arch Microbiol JT - Archives of microbiology JID - 0410427 RN - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid) SB - IM MH - 2,4-Dichlorophenoxyacetic Acid/*metabolism MH - Biodegradation, Environmental MH - Hydrogen-Ion Concentration MH - Oxidation-Reduction MH - Pseudomonas/growth & development/*metabolism MH - *Soil Microbiology EDAT- 1990/01/01 00:00 MHDA- 1990/01/01 00:01 CRDT- 1990/01/01 00:00 PHST- 1990/01/01 00:00 [pubmed] PHST- 1990/01/01 00:01 [medline] PHST- 1990/01/01 00:00 [entrez] AID - 10.1007/BF00276525 [doi] PST - ppublish SO - Arch Microbiol. 1990;154(4):317-22. doi: 10.1007/BF00276525.