PMID- 22450312
OWN - NLM
STAT- MEDLINE
DCOM- 20120622
LR  - 20120423
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 420
IP  - 4
DP  - 2012 Apr 20
TI  - Study of the electrostatic effects of mutations on the surface of 
      dehaloperoxidase-hemoglobin A.
PG  - 733-7
LID - 10.1016/j.bbrc.2012.03.053 [doi]
AB  - Point mutations of dehaloperoxidase-hemoglobin A (DHP A) that affect the surface 
      charge have been prepared to study the interaction between DHP A with its 
      substrate 2,4,6-trichlorophenol (TCP). Kinetic studies of these surface mutations 
      showed a correlation, in which the more positively charged mutants have increased 
      catalytic efficiency compared with wild type DHP A. As a result, the hypothesis 
      of this study is that there is a global electrostatic interaction between DHP A 
      and TCP. The electrostatic nature of substrate binding was further confirmed by 
      the result that kinetic assays of DHP A were affected by ionic strength. 
      Furthermore, isoelectric focusing (IEF) gel study showed that the pI-6.8 for DHP 
      A, which indicates that DHP A has a slight negative charge pH 7, consistent with 
      the kinetic observations.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Zhao, Junjie
AU  - Zhao J
AD  - Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
FAU - Rowe, Jennifer
AU  - Rowe J
FAU - Franzen, Jocelyn
AU  - Franzen J
FAU - He, Chi
AU  - He C
FAU - Franzen, Stefan
AU  - Franzen S
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120317
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Recombinant Proteins)
RN  - 9034-51-9 (Hemoglobin A)
RN  - EC 1.11.1.- (Peroxidases)
SB  - IM
MH  - Animals
MH  - Hemoglobin A/*chemistry/genetics
MH  - Mutagenesis
MH  - Mutation
MH  - Peroxidases/*chemistry/genetics
MH  - Polychaeta/*enzymology
MH  - Recombinant Proteins/chemistry/genetics
MH  - *Static Electricity
EDAT- 2012/03/28 06:00
MHDA- 2012/06/23 06:00
CRDT- 2012/03/28 06:00
PHST- 2012/03/02 00:00 [received]
PHST- 2012/03/12 00:00 [accepted]
PHST- 2012/03/28 06:00 [entrez]
PHST- 2012/03/28 06:00 [pubmed]
PHST- 2012/06/23 06:00 [medline]
AID - S0006-291X(12)00500-1 [pii]
AID - 10.1016/j.bbrc.2012.03.053 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Apr 20;420(4):733-7. doi: 
      10.1016/j.bbrc.2012.03.053. Epub 2012 Mar 17.