PMID- 22453409
OWN - NLM
STAT- MEDLINE
DCOM- 20120801
LR  - 20181023
IS  - 1094-4087 (Electronic)
IS  - 1094-4087 (Linking)
VI  - 20
IP  - 7
DP  - 2012 Mar 26
TI  - Drift estimation for single marker switching based imaging schemes.
PG  - 7274-89
LID - 10.1364/OE.20.007274 [doi]
AB  - In recent years, the diffraction barrier in fluorescence imaging has been broken 
      and optical nanoscopes now routinely image with resolutions of down to 20 nm, an 
      improvement of more than 10 fold. Because this allows imaging much smaller 
      features and because all super-resolution approaches trade off speed for spatial 
      resolution, mechanical instabilities of the microscopes become a limiting factor. 
      Here, we propose a fully data-driven statistical registration method for drift 
      detection and drift correction for single marker switching (SMS) imaging schemes, 
      including a guideline for parameter choice and quality checks of the drift 
      analysis. The necessary assumptions about the drift are minimal, allowing a 
      model-free approach, but more specific models can easily be integrated. We 
      determine the resulting performance on standard SMS measurements and show that 
      the drift determination can be routinely brought to the range of precision 
      achievable by fiducial marker-tracking methods.
FAU - Geisler, Claudia
AU  - Geisler C
AD  - Department of Optical Nanoscopy, Laser-Laboratory-Gottingen, Hans-Adolf-Krebs-Weg 
      1, D-37077 Gottingen, Germany.
FAU - Hotz, Thomas
AU  - Hotz T
FAU - Schonle, Andreas
AU  - Schonle A
FAU - Hell, Stefan W
AU  - Hell SW
FAU - Munk, Axel
AU  - Munk A
FAU - Egner, Alexander
AU  - Egner A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Opt Express
JT  - Optics express
JID - 101137103
SB  - IM
MH  - *Artifacts
MH  - *Fiducial Markers
MH  - Image Enhancement/*instrumentation
MH  - Image Interpretation, Computer-Assisted/*methods
MH  - Microscopy/*instrumentation
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2012/03/29 06:00
MHDA- 2012/08/02 06:00
CRDT- 2012/03/29 06:00
PHST- 2012/03/29 06:00 [entrez]
PHST- 2012/03/29 06:00 [pubmed]
PHST- 2012/08/02 06:00 [medline]
AID - 230391 [pii]
AID - 10.1364/OE.20.007274 [doi]
PST - ppublish
SO  - Opt Express. 2012 Mar 26;20(7):7274-89. doi: 10.1364/OE.20.007274.