PMID- 22455145
OWN - NLM
STAT- MEDLINE
DCOM- 20120806
LR  - 20131121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 43
IP  - 1
DP  - 2012 Jan
TI  - [Determination of canthaxanthin and astaxanthin in egg yolks by reversed phase 
      high performance liquid chromatography with diode array detection].
PG  - 113-7
AB  - OBJECTIVE: A method using reversed phase high performance liquid chromatography 
      (RP-HPLC) coupled with diode array detector (DAD) was developed for the 
      simultaneous determination of canthaxanthin and astaxanthin in egg yolks. 
      METHODS: Samples were extracted with acetonitrile in ultrasonic bath for 20 
      minutes and then purified by freezing-lipid filtration and solid phase extraction 
      (SPE). After being vaporized to dryness by nitrogen blowing and made up to volume 
      with methanol, the extract solution was chromatographically separated in C18 
      column with a unitary mobile phase consisting of acetonitrile. The proposed 
      method was validated in terms of linearity, precision, accuracy, and limit of 
      detection (LOD). RESULT: Regression analysis revealed a good linearity between 
      peak area of each analyte and its concentration (r > or = 0.998). The intra- and 
      inter-day relative standard deviations (RSDs) were less than 3.6% and 5.2%, 
      respectively. LODs of canthaxanthin and astaxanthin were 0.035 and 0.027 
      microg/mL (S/N = 3). The average recoveries of canthaxanthin and astaxanthin were 
      91.5% and 88.7%. CONCLUSION: The proposed method is simple, fast and easy to 
      apply.
FAU - He, Kang-Hao
AU  - He KH
AD  - Department of Sanitary Technology, West China Public Health School, Sichuan 
      University, Chengdu 610041, China.
FAU - Zou, Xiao-Li
AU  - Zou XL
FAU - Liu, Xiang
AU  - Liu X
FAU - Zeng, Hong-Yan
AU  - Zeng HY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Food Coloring Agents)
RN  - 0 (Xanthophylls)
RN  - 4C3C6403MU (Canthaxanthin)
RN  - 8XPW32PR7I (astaxanthine)
SB  - IM
MH  - Animals
MH  - Canthaxanthin/*analysis
MH  - Chromatography, High Pressure Liquid/methods
MH  - Chromatography, Reverse-Phase/methods
MH  - Egg Yolk/*chemistry
MH  - Food Coloring Agents/*analysis
MH  - Food Contamination/*analysis
MH  - Xanthophylls/analysis
EDAT- 2012/03/30 06:00
MHDA- 2012/08/07 06:00
CRDT- 2012/03/30 06:00
PHST- 2012/03/30 06:00 [entrez]
PHST- 2012/03/30 06:00 [pubmed]
PHST- 2012/08/07 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Jan;43(1):113-7.