PMID- 22456337
OWN - NLM
STAT- MEDLINE
DCOM- 20120914
LR  - 20211021
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 11
IP  - 8
DP  - 2012 Apr 15
TI  - Cyclin-dependent kinase 5 regulates E2F transcription factor through 
      phosphorylation of Rb protein in neurons.
PG  - 1603-10
LID - 10.4161/cc.20009 [doi]
AB  - Recent studies have shown the involvement of cyclin-dependent kinase 5 (Cdk5) in 
      cell cycle regulation in postmitotic neurons. In this study, we demonstrate that 
      Cdk5 and its co-activator p35 were detected in the nuclear fraction in neurons 
      and Cdk5/p35 phosphorylated retinoblastoma (Rb) protein, a key protein 
      controlling cell cycle re-entry. Cdk5/p35 phosphorylates Rb at the sites similar 
      to those phosphorylated by Cdk4 and Cdk2. Furthermore, increased Cdk5 activity 
      elevates activity of E2F transcription factor, which can trigger cell cycle 
      re-entry, leading to neuronal cell death. A normal Cdk5 activity in neurons did 
      not induce E2F activation, suggesting that Cdk5 does not induce cell cycle 
      re-entry under normal conditions. Taken together, these results indicate that 
      Cdk5 can regulate cell cycle by its ability to phosphorylate Rb. Most 
      importantly, increased Cdk5 activity induces cell cycle re-entry, which is 
      especially detrimental for survival of postmitotic neurons.
FAU - Futatsugi, Akira
AU  - Futatsugi A
AD  - Functional Genomics Section, Laboratory of Cell and Developmental Biology, 
      National Institute of Dental and Craniofacial Research, National Institutes of 
      Health, Bethesda, MD, USA.
FAU - Utreras, Elias
AU  - Utreras E
FAU - Rudrabhatla, Parvathi
AU  - Rudrabhatla P
FAU - Jaffe, Howard
AU  - Jaffe H
FAU - Pant, Harish C
AU  - Pant HC
FAU - Kulkarni, Ashok B
AU  - Kulkarni AB
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20120415
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (E2F Transcription Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphopeptides)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (neuronal Cdk5 activator (p25-p35))
RN  - EC 2.7.11.1 (Cyclin-Dependent Kinase 5)
SB  - IM
CIN - Cell Cycle. 2012 Jun 1;11(11):2049. PMID: 22622087
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cyclin-Dependent Kinase 5/deficiency/genetics/*metabolism
MH  - E2F Transcription Factors/*metabolism
MH  - Mass Spectrometry
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/deficiency/genetics/metabolism
MH  - Neurons/*metabolism
MH  - Phosphopeptides/analysis
MH  - Phosphorylation
MH  - Retinoblastoma Protein/*metabolism
PMC - PMC3341229
EDAT- 2012/03/30 06:00
MHDA- 2012/09/15 06:00
PMCR- 2013/04/15
CRDT- 2012/03/30 06:00
PHST- 2012/03/30 06:00 [entrez]
PHST- 2012/03/30 06:00 [pubmed]
PHST- 2012/09/15 06:00 [medline]
PHST- 2013/04/15 00:00 [pmc-release]
AID - 20009 [pii]
AID - 2011CC3869R [pii]
AID - 10.4161/cc.20009 [doi]
PST - ppublish
SO  - Cell Cycle. 2012 Apr 15;11(8):1603-10. doi: 10.4161/cc.20009. Epub 2012 Apr 15.