PMID- 22459196
OWN - NLM
STAT- MEDLINE
DCOM- 20120809
LR  - 20161125
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 33
IP  - 18
DP  - 2012 Jun
TI  - The effects of sub-lethal concentrations of silver nanoparticles on inflammatory 
      and stress genes in human macrophages using cDNA microarray analysis.
PG  - 4690-9
LID - 10.1016/j.biomaterials.2012.03.006 [doi]
AB  - Because of the limited information on size-dependent particle-mediated effects, 
      the present study was conducted to determine if the changes in induced protein 
      expression between 5 nm silver nanoparticles and 100 nm particles after exposure 
      to sub-lethal concentrations. A total of 28,000 cDNA profiles were screened using 
      5 nm silver nanoparticles and 100 nm silver nanoparticles in a macrophage cell 
      line. Based on results obtained from cDNA microarray we also assessed protein 
      levels of hemeoxygenase-1 (HO-1), heat shock protein-70 (HSP-70) and 
      interleukin-8 (IL-8), which were shown to significantly increase. Together with 
      results obtained using N-acetylcystein (NAC), we were able to clearly show that 
      low level and early stage exposure to 5 nm silver nanoparticles, but not 100 nm, 
      induces expression of IL-8 as well as stress genes against reactive oxygen 
      species (ROS). Therefore, we provide important data to understand and identify 
      the early effects of silver nanoparticles on the immune system.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Lim, Dae-Hyoun
AU  - Lim DH
AD  - Department of Microbiology, Brain Korea 21 Project for Medical Science, Institute 
      for Immunology and Immunological Diseases, Yonsei University College of Medicine, 
      50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
FAU - Jang, Jiyoung
AU  - Jang J
FAU - Kim, Seungjae
AU  - Kim S
FAU - Kang, Taegyeong
AU  - Kang T
FAU - Lee, Kangtaek
AU  - Lee K
FAU - Choi, In-Hong
AU  - Choi IH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120327
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (Reactive Oxygen Species)
RN  - 3M4G523W1G (Silver)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - HSP70 Heat-Shock Proteins/metabolism
MH  - Heme Oxygenase-1/metabolism
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - Macrophages/*drug effects/*metabolism
MH  - Metal Nanoparticles/adverse effects/chemistry/*therapeutic use/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Reactive Oxygen Species/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Silver/adverse effects/chemistry/*therapeutic use
EDAT- 2012/03/31 06:00
MHDA- 2012/08/10 06:00
CRDT- 2012/03/31 06:00
PHST- 2012/01/27 00:00 [received]
PHST- 2012/03/03 00:00 [accepted]
PHST- 2012/03/31 06:00 [entrez]
PHST- 2012/03/31 06:00 [pubmed]
PHST- 2012/08/10 06:00 [medline]
AID - S0142-9612(12)00278-5 [pii]
AID - 10.1016/j.biomaterials.2012.03.006 [doi]
PST - ppublish
SO  - Biomaterials. 2012 Jun;33(18):4690-9. doi: 10.1016/j.biomaterials.2012.03.006. 
      Epub 2012 Mar 27.