PMID- 22459409
OWN - NLM
STAT- MEDLINE
DCOM- 20120608
LR  - 20120423
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 116-117
DP  - 2012 Jul 15
TI  - Natural mixtures of persistent organic pollutants (POPs) suppress ovarian 
      follicle development, liver vitellogenin immunostaining and hepatocyte 
      proliferation in female zebrafish (Danio rerio).
PG  - 16-23
LID - 10.1016/j.aquatox.2012.02.031 [doi]
AB  - Persistent organic pollutants such as polychlorinated biphenyls (PCBs), 
      polybrominated diphenyl ethers (PBDEs) and dichlorodiphenyltrichloroethane (DDT) 
      are present in high concentrations in livers of burbot (Lota lota) in Lake Mjosa, 
      Norway. In order to assess effects of such pollutants on fish gonadal morphology, 
      female zebrafish were exposed in two generations by food to mixtures of 
      pollutants extracted from livers of burbot from Lake Mjosa (high and low dose) 
      and Lake Losna, which represents background pollution, and compared to a control 
      group. Ovarian follicle counts detected a significant decrease in late 
      vitellogenic follicle stages in fish exposed to the Losna and the high 
      concentrations of Mjosa mixtures in fish from the first generation. In addition, 
      proliferation of granulosa cells, visualized by immunohistochemistry against 
      proliferating cell nuclear antigen (PCNA), was decreased in all exposure groups 
      in either early or late vitellogenic follicle stages compared to control. This 
      was accompanied by increased apoptosis of granulosa cells. There was a decrease 
      in proliferation of liver hepatocytes with exposure to both Mjosa mixtures. In 
      addition, immunopositivity for vitellogenin in the liver was significantly lower 
      in the Mjosa high group than in the control group. When analysing effects of 
      parental exposure, fish with parents exposed to Mjosa high mixture had 
      significantly higher numbers of perinucleolar follicles than fish with control 
      parents. We conclude that long-term exposure of a real-life mixture of pollutants 
      containing high- and background levels of chemicals supress ovarian follicle 
      development, liver vitellogenin immunostaining intensity and hepatocyte 
      proliferation in the zebrafish model.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Kraugerud, Marianne
AU  - Kraugerud M
AD  - Dept. of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary 
      Science, POB 8146 Dep., 0033 Oslo, Norway. Marianne.Kraugerud@nvh.no
FAU - Doughty, Richard William
AU  - Doughty RW
FAU - Lyche, Jan L
AU  - Lyche JL
FAU - Berg, Vidar
AU  - Berg V
FAU - Tremoen, Nina H
AU  - Tremoen NH
FAU - Alestrom, Peter
AU  - Alestrom P
FAU - Aleksandersen, Mona
AU  - Aleksandersen M
FAU - Ropstad, Erik
AU  - Ropstad E
LA  - eng
PT  - Journal Article
DEP - 20120305
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Vitellogenins)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Hepatocytes/cytology/*drug effects
MH  - Liver/*drug effects
MH  - Ovarian Follicle/*drug effects
MH  - Vitellogenins/*metabolism
MH  - Water Pollutants, Chemical/*toxicity
MH  - Zebrafish/*physiology
EDAT- 2012/03/31 06:00
MHDA- 2012/06/09 06:00
CRDT- 2012/03/31 06:00
PHST- 2012/01/18 00:00 [received]
PHST- 2012/02/21 00:00 [revised]
PHST- 2012/02/25 00:00 [accepted]
PHST- 2012/03/31 06:00 [entrez]
PHST- 2012/03/31 06:00 [pubmed]
PHST- 2012/06/09 06:00 [medline]
AID - S0166-445X(12)00089-6 [pii]
AID - 10.1016/j.aquatox.2012.02.031 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2012 Jul 15;116-117:16-23. doi: 10.1016/j.aquatox.2012.02.031. 
      Epub 2012 Mar 5.