PMID- 22459824
OWN - NLM
STAT- MEDLINE
DCOM- 20120810
LR  - 20131121
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 95
IP  - 4
DP  - 2012 Apr
TI  - Alterations of fatty acid beta-oxidation capability in the liver of ketotic cows.
PG  - 1759-66
LID - 10.3168/jds.2011-4580 [doi]
AB  - Dairy cows are highly susceptible to ketosis after parturition. In the present 
      study, we evaluated the expression of fatty acid beta-oxidation-related enzymes in 
      the liver of ketotic (n=6) and nonketotic (n=6) cows. Serum levels of 
      nonesterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), and glucose were 
      determined by using standard biochemical techniques. The mRNA abundance and 
      protein content of acyl-CoA synthetase long-chain (ACSL), carnitine 
      palmitoyltransferase I (CPT I), carnitine palmitoyltransferase II (CPT II), 
      acyl-CoA dehydrogenase long chain (ACADL), 3-hydroxy-3-methylglutaryl-CoA 
      synthase (HMGCS), and acetyl-CoA carboxylase (ACC) were evaluated by real-time 
      PCR and ELISA. We found that serum glucose levels were lower in ketotic cows than 
      in nonketotic cows, but serum BHBA and NEFA concentrations were higher. Messenger 
      RNA and protein levels of ACSL were significantly higher in livers of ketotic 
      cows than those in nonketotic cows. In contrast, mRNA levels of CPT I and mRNA 
      and protein levels of CPT II, ACADL, HMGCS, and ACC were decreased in the liver 
      of ketotic cows. Serum NEFA concentration positively correlated with ACSL protein 
      levels and negatively correlated with protein levels of CPT II, HMGCS, ACADL, and 
      ACC. In addition, serum BHBA concentration negatively correlated with protein 
      levels of CPT II, HMGCS, and ACADL. Overall, fatty acid beta-oxidation capability 
      was altered in the liver of ketotic compared with nonketotic cows. Furthermore, 
      high serum NEFA and BHBA concentrations play key roles in affecting pathways of 
      fatty acid metabolism in the liver.
CI  - Copyright (c) 2012 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Li, P
AU  - Li P
AD  - Department of Clinical Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China.
FAU - Li, X B
AU  - Li XB
FAU - Fu, S X
AU  - Fu SX
FAU - Wu, C C
AU  - Wu CC
FAU - Wang, X X
AU  - Wang XX
FAU - Yu, G J
AU  - Yu GJ
FAU - Long, M
AU  - Long M
FAU - Wang, Z
AU  - Wang Z
FAU - Liu, G W
AU  - Liu GW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (Blood Glucose)
RN  - 0 (Fatty Acids)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (RNA, Messenger)
RN  - EC 1.3.- (Acyl-CoA Dehydrogenases)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
RN  - EC 2.3.3.10 (Hydroxymethylglutaryl-CoA Synthase)
RN  - EC 6.2.1.- (Coenzyme A Ligases)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid/blood
MH  - Acetyl-CoA Carboxylase/analysis/genetics
MH  - Acyl-CoA Dehydrogenases/analysis/genetics
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Carnitine O-Palmitoyltransferase/analysis/genetics
MH  - Cattle
MH  - Cattle Diseases/*enzymology
MH  - Coenzyme A Ligases/analysis/genetics
MH  - Fatty Acids/*metabolism
MH  - Fatty Acids, Nonesterified/blood
MH  - Female
MH  - Hydroxymethylglutaryl-CoA Synthase/analysis/genetics
MH  - Ketosis/enzymology/*veterinary
MH  - Liver/*enzymology
MH  - Oxidation-Reduction
MH  - Puerperal Disorders/enzymology/*veterinary
MH  - RNA, Messenger/analysis
EDAT- 2012/03/31 06:00
MHDA- 2012/08/11 06:00
CRDT- 2012/03/31 06:00
PHST- 2011/05/27 00:00 [received]
PHST- 2011/12/14 00:00 [accepted]
PHST- 2012/03/31 06:00 [entrez]
PHST- 2012/03/31 06:00 [pubmed]
PHST- 2012/08/11 06:00 [medline]
AID - S0022-0302(12)00141-5 [pii]
AID - 10.3168/jds.2011-4580 [doi]
PST - ppublish
SO  - J Dairy Sci. 2012 Apr;95(4):1759-66. doi: 10.3168/jds.2011-4580.