PMID- 22460985
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20211021
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 174
IP  - 3
DP  - 2012 Sep
TI  - Experimental model of arthritis induced by Paracoccidioides brasiliensis in rats.
PG  - 187-91
LID - 10.1007/s11046-012-9537-8 [doi]
AB  - Paracoccidioidomycosis (PCM), a disease caused by the fungus Paracoccidioides 
      brasiliensis (Pb), is highly prevalent in Brazil, where it is the principal cause 
      of death by systemic mycoses. The disease primarily affects men aged 30-50 year 
      old and usually starts as a pulmonary focus and then may spread to other organs 
      and systems, including the joints. The present study aimed to develop an 
      experimental model of paracoccidioidomycotic arthritis. Two-month-old male Wistar 
      rats (n = 48) were used, divided in 6 groups: test groups EG/15 and EG/45 
      (received one dose of 100 mul of saline containing 10(5) Pb viable yeasts in the 
      knee); heat killed Pb-group HK/15 and HK/45 (received a suspension of 10(5) Pb 
      nonviable yeasts in the knee) and control groups CG/15 and CG/45 (received only 
      sterile saline in the knee). The rats were killed 15 and 45 days postinoculation. 
      In contrast with the control rats, the histopathology of the joints of rats of 
      the test groups (EG/15 and EG/45) revealed a picture of well-established PCM 
      arthritis characterized by extensive sclerosing granulomatous inflammation with 
      numerous multiple budding fungal cells. The X-ray examination revealed joint 
      alterations in these groups. Only metabolic active fungi evoked inflammation. The 
      experimental model was able to induce fungal arthritis in the knees of the rats 
      infected with metabolic active P. brasiliensis. The disease tended to be 
      regressive and restrained by the immune system. No evidence of fungal 
      dissemination to the lungs was observed.
FAU - Loth, Eduardo Alexandre
AU  - Loth EA
AD  - Hospital Universitario do Oeste do Parana, Bairro: Santo Onofre, Cascavel, PR, 
      Brazil.
FAU - Biazin, Samia Khalil
AU  - Biazin SK
FAU - Paula, Claudete Rodrigues
AU  - Paula CR
FAU - Simao, Rita de Cassia Garcia
AU  - Simao Rde C
FAU - de Franco, Marcello Fabiano
AU  - de Franco MF
FAU - Puccia, Rosana
AU  - Puccia R
FAU - Gandra, Rinaldo Ferreira
AU  - Gandra RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120330
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
SB  - IM
MH  - Animals
MH  - Arthritis/microbiology/*pathology
MH  - Arthrography
MH  - *Disease Models, Animal
MH  - Histocytochemistry
MH  - Joints/pathology
MH  - Male
MH  - Paracoccidioides/*pathogenicity
MH  - Paracoccidioidomycosis/microbiology/*pathology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2012/03/31 06:00
MHDA- 2012/11/02 06:00
CRDT- 2012/03/31 06:00
PHST- 2012/01/08 00:00 [received]
PHST- 2012/03/14 00:00 [accepted]
PHST- 2012/03/31 06:00 [entrez]
PHST- 2012/03/31 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
AID - 10.1007/s11046-012-9537-8 [doi]
PST - ppublish
SO  - Mycopathologia. 2012 Sep;174(3):187-91. doi: 10.1007/s11046-012-9537-8. Epub 2012 
      Mar 30.