PMID- 2246148
OWN - NLM
STAT- MEDLINE
DCOM- 19910108
LR  - 20171213
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 69
IP  - 3
DP  - 1990 Sep
TI  - Late pulmonary allergic responses in actively but not passively IgE-sensitized 
      rats.
PG  - 1012-21
AB  - Previous studies suggested that although rats that were passively sensitized 
      [monoclonal murine immunoglobulin E (IgE)] would respond to pulmonary antigen 
      challenge with an immediate increase in resistance, they exhibited no late 
      increases in resistance, unlike late changes in rats actively sensitized to 
      preferentially produce IgE antibody. We hypothesized that passively sensitized 
      rats also would not develop antigen-induced pulmonary inflammation. In a blinded 
      protocol we compared immediate responses and pulmonary resistance and 
      inflammation at 8, 19 and 24 h after challenge with placebo antigen, with 
      dinitrophenol-bovine serum albumin (DNP-BSA) to elicit a passively sensitized 
      response, or with ovalbumin (OA) to elicit an actively sensitized response. 
      Despite similar immediate responses to OA and DNP-BSA, only the rats challenged 
      with OA had marked inflammatory changes and a significant incidence of late 
      elevations in resistance. Inflammation scores and lung resistance were 
      significantly correlated only in the OA group. We also observed that anesthesia 
      with fentanyl/droperidol significantly attenuated the immediate but not the late 
      responses to antigen challenge, compared with rats anesthetized with ketamine. We 
      conclude that IgE-mediated immediate responses to pulmonary antigen challenge are 
      insufficient, and may be unnecessary, to initiate antigen-induced late 
      inflammatory changes.
FAU - Sorkness, R
AU  - Sorkness R
AD  - Department of Medicine and Pediatrics, School of Medicine, University of 
      Wisconsin, Madison 53706.
FAU - Johns, K
AU  - Johns K
FAU - Castleman, W L
AU  - Castleman WL
FAU - Lemanske, R F Jr
AU  - Lemanske RF Jr
LA  - eng
GR  - 1-K08-HL-01147-01/HL/NHLBI NIH HHS/United States
GR  - AI-10404/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Dinitrophenols)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Airway Resistance/physiology
MH  - Animals
MH  - Dinitrophenols/pharmacology
MH  - Immunization
MH  - Immunization, Passive
MH  - Immunoglobulin E/*immunology
MH  - Ovalbumin/immunology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Respiratory Function Tests
MH  - Respiratory Hypersensitivity/*physiopathology
MH  - Serum Albumin, Bovine/immunology
EDAT- 1990/09/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
AID - 10.1152/jappl.1990.69.3.1012 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 1990 Sep;69(3):1012-21. doi: 10.1152/jappl.1990.69.3.1012.