PMID- 22462635
OWN - NLM
STAT- MEDLINE
DCOM- 20121002
LR  - 20220318
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 10
DP  - 2012 Apr 2
TI  - The effect of bone marrow microenvironment on the functional properties of the 
      therapeutic bone marrow-derived cells in patients with acute myocardial 
      infarction.
PG  - 66
LID - 10.1186/1479-5876-10-66 [doi]
AB  - BACKGROUND: Treatment of acute myocardial infarction with stem cell 
      transplantation has achieved beneficial effects in many clinical trials. The bone 
      marrow microenvironment of ST-elevation myocardial infarction (STEMI) patients 
      has never been studied even though myocardial infarction is known to cause an 
      imbalance in the acid-base status of these patients. The aim of this study was to 
      assess if the blood gas levels in the bone marrow of STEMI patients affect the 
      characteristics of the bone marrow cells (BMCs) and, furthermore, do they 
      influence the change in cardiac function after autologous BMC transplantation. 
      The arterial, venous and bone marrow blood gas concentrations were also compared. 
      METHODS: Blood gas analysis of the bone marrow aspirate and peripheral blood was 
      performed for 27 STEMI patients receiving autologous stem cell therapy after 
      percutaneous coronary intervention. Cells from the bone marrow aspirate were 
      further cultured and the bone marrow mesenchymal stem cell (MSC) proliferation 
      rate was determined by MTT assay and the MSC osteogenic differentiation capacity 
      by alkaline phosphatase (ALP) activity assay. All the patients underwent a 
      2D-echocardiography at baseline and 4 months after STEMI. RESULTS: As expected, 
      the levels of pO(2), pCO(2), base excess and HCO(3) were similar in venous blood 
      and bone marrow. Surprisingly, bone marrow showed significantly lower pH and 
      Na(+) and elevated K(+) levels compared to arterial and venous blood. There was a 
      positive correlation between the bone marrow pCO(2) and HCO(3) levels and MSC 
      osteogenic differentiation capacity. In contrast, bone marrow pCO(2) and HCO(3) 
      levels displayed a negative correlation with the proliferation rate of MSCs. 
      Patients with the HCO(3) level below the median value exhibited a more marked 
      change in LVEF after BMC treatment than patients with HCO(3) level above the 
      median (11.13 +/- 8.07% vs. 2.67 +/- 11.89%, P = 0.014). CONCLUSIONS: Low bone marrow 
      pCO(2) and HCO(3) levels may represent the optimal environment for BMCs in terms 
      of their efficacy in autologous stem cell therapy in STEMI patients.
FAU - Miettinen, Johanna A
AU  - Miettinen JA
AD  - Department of Internal Medicine, Institute of Clinical Medicine, University of 
      Oulu, Oulu FIN-90014, Finland. johanna.miettinen@oulu.fi
FAU - Salonen, Riikka J
AU  - Salonen RJ
FAU - Ylitalo, Kari
AU  - Ylitalo K
FAU - Niemela, Matti
AU  - Niemela M
FAU - Kervinen, Kari
AU  - Kervinen K
FAU - Saily, Marjaana
AU  - Saily M
FAU - Koistinen, Pirjo
AU  - Koistinen P
FAU - Savolainen, Eeva-Riitta
AU  - Savolainen ER
FAU - Makikallio, Timo H
AU  - Makikallio TH
FAU - Huikuri, Heikki V
AU  - Huikuri HV
FAU - Lehenkari, Petri
AU  - Lehenkari P
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20120402
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Gas Analysis
MH  - Bone Marrow/blood supply
MH  - Bone Marrow Cells/chemistry/metabolism/pathology/*physiology
MH  - Bone Marrow Transplantation/*physiology
MH  - Cells, Cultured
MH  - Cellular Microenvironment/*physiology
MH  - Double-Blind Method
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Myocardial Infarction/diagnosis/pathology/*physiopathology/therapy
MH  - Pilot Projects
MH  - Prognosis
MH  - Stroke Volume/physiology
MH  - Transplantation, Autologous
MH  - Treatment Outcome
MH  - Young Adult
PMC - PMC3366890
EDAT- 2012/04/03 06:00
MHDA- 2012/10/04 06:00
PMCR- 2012/04/02
CRDT- 2012/04/03 06:00
PHST- 2011/11/28 00:00 [received]
PHST- 2012/04/02 00:00 [accepted]
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/10/04 06:00 [medline]
PHST- 2012/04/02 00:00 [pmc-release]
AID - 1479-5876-10-66 [pii]
AID - 10.1186/1479-5876-10-66 [doi]
PST - epublish
SO  - J Transl Med. 2012 Apr 2;10:66. doi: 10.1186/1479-5876-10-66.