PMID- 22463766
OWN - NLM
STAT- MEDLINE
DCOM- 20120925
LR  - 20211021
IS  - 1746-1596 (Electronic)
IS  - 1746-1596 (Linking)
VI  - 7
DP  - 2012 Mar 30
TI  - Human telomerase RNA component (hTERC) gene amplification detected by FISH in 
      precancerous lesions and carcinoma of the larynx.
PG  - 34
LID - 10.1186/1746-1596-7-34 [doi]
AB  - BACKGROUND: Gain of 3q26 is frequently observed in squamous cell carcinomas of 
      mucosal origin, including those originating in the head and neck region. The 
      human telomerase RNA component (hTERC) gene, which is located on chromosome 3q26, 
      encodes for an RNA subunit of telomerase that maintains the length of telomeres 
      through cellular divisions, and is activated in malignant diseases. The present 
      study was designed to detect hTERC amplification in laryngeal lesions and 
      evaluate whether this might serve as a supportive biomarker in histopathological 
      analysis for in the diagnosis of laryngeal lesions. METHODS: Fluorescent in situ 
      hybridization (FISH) was applied on formalin-fixed paraffin-embedded blocks of 93 
      laryngeal specimens, including 14 normal epithelium (NE), 15 mild dysplasia (Md), 
      18 moderate dysplasia (MD), 16 severe dysplasia (SD), 9 carcinoma in situ (CIS), 
      and 21 invasive carcinoma (IC)). RESULTS: By histopathologic examination, hTERC 
      amplification rates in NE, Md, MD, SD, CIS and IC cases were 0% (0/14), 13.33% 
      (2/15), 72.22% (13/18), 81.25% (13/16), 100% (9/9) and 100% (21/21), 
      respectively. Amplification of hTERC was significantly associated with 
      histopathologic diagnosis (P < 0.0001). The percentage of hTERC amplification in 
      patients with MD, SD, CIS, and IC was significantly higher than those with NE or 
      Md (P < 0.0001). The number of cells with abnormal signals increased and the 
      abnormal signal patterns were diversified with increasing severity of laryngeal 
      dysplasia (P < 0.0001). CONCLUSIONS: The hTERC amplification is important in the 
      development of laryngeal squamous cell carcinoma (LSCC). FISH detection of hTERC 
      amplification may provide an effective approach in conjunction with 
      histopathologic evaluation for differential diagnosis of laryngeal lesions. 
      VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: 
      http://www.diagnosticpathology.diagnomx.eu/vs/2226606266791985.
FAU - Liu, Yu
AU  - Liu Y
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, 100730, China.
FAU - Dong, Xiao-li
AU  - Dong XL
FAU - Tian, Cheng
AU  - Tian C
FAU - Liu, Hong-gang
AU  - Liu HG
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120330
PL  - England
TA  - Diagn Pathol
JT  - Diagnostic pathology
JID - 101251558
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Carcinoma, Squamous Cell/diagnosis/*metabolism/pathology
MH  - Diagnosis, Differential
MH  - Epithelium/metabolism/pathology
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Laryngeal Neoplasms/diagnosis/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Precancerous Conditions/diagnosis/*metabolism/pathology
MH  - RNA/*genetics/*metabolism
MH  - Telomerase/*genetics/*metabolism
PMC - PMC3359179
EDAT- 2012/04/03 06:00
MHDA- 2012/09/26 06:00
PMCR- 2012/03/30
CRDT- 2012/04/03 06:00
PHST- 2012/01/25 00:00 [received]
PHST- 2012/03/30 00:00 [accepted]
PHST- 2012/04/03 06:00 [entrez]
PHST- 2012/04/03 06:00 [pubmed]
PHST- 2012/09/26 06:00 [medline]
PHST- 2012/03/30 00:00 [pmc-release]
AID - 1746-1596-7-34 [pii]
AID - 10.1186/1746-1596-7-34 [doi]
PST - epublish
SO  - Diagn Pathol. 2012 Mar 30;7:34. doi: 10.1186/1746-1596-7-34.